Translator Disclaimer
1 September 2008 Molecular Cloning and Developmental Expression of a Hyaluronan and Proteoglycan Link Protein Gene, crtl1/hapln1, in Zebrafish
Author Affiliations +
Abstract

The proteoglycan aggregate of the cartilage is composed of aggrecan, link protein (LP), and hyaluronan, providing resistance to compression in joints and cartilage structures. To further understand the function of LP during the process of chondrogenesis and bone formation in zebrafish, we cloned the zebrafish cDNA for hyaluronan and proteoglycan link protein 1 (crtl1/hapln1) and examined the expression of the gene during embryogenesis using in-situ hybridization. crtl1/hapln1 expression is first observed in the adaxial cells at the bud- stage. Throughout somitogenesis, crtl1/hapln1 is expressed in the sclerotomes, floor plate, and hypochord. In addition, crtl1/hapln1 is expressed in rhombomeres 3 and 5, pharyngeal arches, telecephalon, otic vesicles, and pectral fins. During chondrocranial/skull formation, crtl1/hapln1 expression is highest at around 4 dpf and is colocalized with aggrecan in the cartilaginous arches and with dermacan in the dermal bones.

Jeong Suk Kang, Yasuhiko Kawakami, Yoko Bekku, Yoshifumi Ninomiya, Juan Carlos Izpisúa Belmonte, and Toshitaka Oohashi "Molecular Cloning and Developmental Expression of a Hyaluronan and Proteoglycan Link Protein Gene, crtl1/hapln1, in Zebrafish," Zoological Science 25(9), 912-918, (1 September 2008). https://doi.org/10.2108/zsj.25.912
Received: 24 April 2008; Accepted: 1 June 2008; Published: 1 September 2008
JOURNAL ARTICLE
7 PAGES


SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top