1 March 2009 Reprobing Multicolor Fish Preparations in Lepidopteran Chromosome
Fukashi Shibata, Ken Sahara, Yota Naito, Yuji Yasukochi
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Multicolor fluorescence in-situ hybridization (FISH) and subsequent reprobing of chromosome preparations increase the number of chromosomes and/or anchor loci on the chromosomes simultaneously identified. Reprobing techniques have been widely applied to chromosomes of vertebrates and plants. We have developed a novel reprobing protocol that utilizes multicolor FISH and bacterial artificial chromosome (BAC) probes to examine chromosome preparations in a model lepidopteran species, the silkworm, Bombyx mori. With standard two-color BAC-FISH, routinely used to map genes on B. mori chromosomes, we could localize only two probes on one preparation, whereas our new protocol combining five-color BAC-FISH and preparation reprobing enabled us to simultaneously map 10 probes, as demonstrated with the Bombyx Z chromosome. The improved BAC-FISH technique will facilitate karyotyping and synteny analysis in Lepidoptera.

© 2009 Zoological Society of Japan
Fukashi Shibata, Ken Sahara, Yota Naito, and Yuji Yasukochi "Reprobing Multicolor Fish Preparations in Lepidopteran Chromosome," Zoological Science 26(3), 187-190, (1 March 2009). https://doi.org/10.2108/zsj.26.187
Received: 2 November 2008; Accepted: 1 December 2008; Published: 1 March 2009
Bombyx mori
sex chromosome
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