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1 February 2010 Multidimensional Analysis of Uncharacterized Sperm Proteins in Ciona intestinalis: EST-Based Analysis and Functional Immunoscreening of Testis-Expressed Genes
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Abstract
An extensive analysis of testis-expressed genes in the ascidian Ciona intestinalis explored a large number of genes of unknown function. Here we characterized these genes or gene products in a multidimensional manner. We analyzed genes both highly and uniquely expressed in the testis, as expected from the EST analysis. Immunolocalization of these proteins revealed that they are all expressed in sperm. Sperm membrane/matrix proteins play essential roles in cell responses and intracellular signaling at fertilization. By immunoscreening with antisera against the detergentsoluble and membrane fractions of sperm, we isolated 49 potential cDNA clones for membrane/matrix proteins. These included several unidentified genes, including a protein with sequence similarity to mammalian testicular cancer antigen Sp17. These data should facilitate exploration of the functions of uncharacterized sperm proteins and ultimately elucidate new molecular mechanisms in sperm physiology.
© 2010 Zoological Society of Japan
Alu Konno, Potturi Padma, Yuji Ushimaru and Kazuo Inaba "Multidimensional Analysis of Uncharacterized Sperm Proteins in Ciona intestinalis: EST-Based Analysis and Functional Immunoscreening of Testis-Expressed Genes," Zoological Science 27(2), (1 February 2010). https://doi.org/10.2108/zsj.27.204
Received: 13 October 2009; Accepted: 14 November 2009; Published: 1 February 2010
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