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1 October 2011 DNA Sexing of Nipponia nippon by Duplex Polymerase Chain Reaction
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The aim of this study was to develop a rapid, simple, sensitive, and accurate duplex polymerase chain reaction (PCR) to sex Nipponia nippon, a monomorphic bird. Amplification by duplex PCR of a sex-related gene on the female chromosome and the 12S rRNA gene yielded good results using genomic DNA extracted from a feather follicle or the membranes of eggshell samples. To simplify the DNA extraction procedure, a simple boiling method was used. Our simple boiling DNA extraction method produced similar PCR amplification results as when using DNA extracted using TRIzol. Sex determination in the endangered Nipponia nippon is of crucial value to breeding programs. The duplex PCR protocol that we developed provides a simple sex identification method that is based on amplification of a sex-related gene, and we anticipate that it will facilitate effective conservation and management of Nipponia nippon.

© 2011 Zoological Society of Japan
Jie Yeun Park, Ji Hye Lee, Seon A Choi, Keun Jung Kim, Il Beum Lee, and Min Kyu Kim "DNA Sexing of Nipponia nippon by Duplex Polymerase Chain Reaction," Zoological Science 28(10), 740-742, (1 October 2011).
Received: 10 January 2011; Accepted: 1 February 2011; Published: 1 October 2011

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