1 May 2012 Recordings from Cultured Newt Olfactory Receptor Cells
Kyohei Matsumura, Masahiro Matsumoto, Takashi Kurahashi, Hiroko Takeuchi
Author Affiliations +

Freshly dissociated olfactory receptor cells (ORCs) are commonly used in electrophysiological research investigations of the physicochemical mechanisms of olfactory signal transduction. Because the morphology of cultured cells clearly becomes worse over time, the ORCs are examined traditionally within several days after dissociation. However, there has been a major concern that cells are affected soon after dissociation. To gain a better understanding of the reliability of data obtained from solitary cells, we obtained electrical data during the lifetime of single ORCs dissociated from the newt. The time course for the deterioration could be revealed by monitoring the membrane properties during culture. Although the number of living cells that were identified by trypan blue extrusion declined day by day, the remaining cells retained morphology and their fundamental electrical features until day 19. In some cells, the cilia and dendrite were observed until day 21, and the bipolar morphology until day 31. The fundamental features of cell excitation were maintained during culture without showing remarkable changes when they retained morphological features. The results suggest that electrical properties of cells are almost unchanged within several days. Furthermore, the dissociated newt ORCs can be used for several weeks that are almost comparable to the intrinsic lifetime of the ORCs in vivo.

© 2012 Zoological Society of Japan
Kyohei Matsumura, Masahiro Matsumoto, Takashi Kurahashi, and Hiroko Takeuchi "Recordings from Cultured Newt Olfactory Receptor Cells," Zoological Science 29(5), 340-345, (1 May 2012). https://doi.org/10.2108/zsj.29.340
Received: 19 November 2011; Accepted: 1 December 2011; Published: 1 May 2012
lifetime extension
olfactory receptor cell
transduction channels
voltage dependent channels
Get copyright permission
Back to Top