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1 June 2013 Effects of Transient Forebrain Ischemia on the Hippocampus of the Mongolian Gerbil (Meriones unguiculatus): An Immunohistochemical Study
Toshiya Okada, Yusuke Kataoka, Ai Takeshita, Masaki Mino, Hiroshi Morioka, Ken Takeshi Kusakabe, Tomohiro Kondo
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Abstract

In the Mongolian gerbil, bilateral common carotid artery occlusion (BCCAO) for several minutes induces ischemia and delayed neuronal cell death in the CA1 region of the hippocampus due to their incomplete Circle of Willis. In the present study, the expression of fibroblast growth factor 2 (FGF2), its receptors (FGFR1 and FGFR2), glial fibrillary acidic protein (GFAP), and isolectin B4 (ISLB4) was investigated by immunohistochemical and lectin-binding methods after BCCAO was performed for 5 min in gerbils. One day after BCCAO, the pyramidal cells of the CA1 region of the hippocampus showed degenerative changes and lowered expression of FGF2, FGFR1, and FGFR2. Three days after BCCAO, there was an increase in GFAP-positive astrocytes and ISLB4-positive microglial cells. From five to 10 days after BCCAO, intense neuronal cell death in the stria pyramidale of the hippocampal CA1 region was observed, as well as an increase in GFAP-positive astrocytes and decrease in ISLB4-positive microglial cells. These results indicate that transient forebrain ischemia induces neuronal cell death with lowered expression of FGF2 and its receptors, and that the activation of glial cells may not directly lead to neuronal cell death.

© 2013 Zoological Society of Japan
Toshiya Okada, Yusuke Kataoka, Ai Takeshita, Masaki Mino, Hiroshi Morioka, Ken Takeshi Kusakabe, and Tomohiro Kondo "Effects of Transient Forebrain Ischemia on the Hippocampus of the Mongolian Gerbil (Meriones unguiculatus): An Immunohistochemical Study," Zoological Science 30(6), 484-489, (1 June 2013). https://doi.org/10.2108/zsj.30.484
Received: 24 August 2012; Accepted: 1 January 2013; Published: 1 June 2013
JOURNAL ARTICLE
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KEYWORDS
astrocyte
delayed neuronal cell death
FGF2
forebrain ischemia
microglial cell
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