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1 July 2013 Purification and Characterization of a Tachykinin-Like Peptide from Skin Secretions of the Tree Frog, Theloderma kwangsiensis
Hao Zhang, Lin Wei, Chengda Zou, Jeff Jinfeng Bai, Yuzhu Song, Huan Liu
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Abstract

Most amphibians belonging to Rhacophoridae have an arboreal life. A large amount of bioactive peptides have been identified from amphibian skin, but none from amphibians belonging to Rhacophoridae have been reported to date. A tachykinin-like peptide, tachykinin-Thel, was purified and characterized from skin secretions of the tree frog, Theloderma kwangsiensis. Its primary structure was determined as KPSPDRFYGLM-NH2 by Edman degradation and mass spectrometry analysis. cDNA sequence encoding the precursor of tachykinin-Thel was cloned from the skin cDNA library. Tachykinin-Thel induced the contraction of isolated ileum smooth muscle in a dose-dependent manner. The current work reported a bioactive peptide, tachykinin-Thel, from Rhacophoridae amphibians and confirmed the presence of tachykinin-like peptide in the skin of the tree frog, which may facilitate their arboreal life.

© 2013 Zoological Society of Japan
Hao Zhang, Lin Wei, Chengda Zou, Jeff Jinfeng Bai, Yuzhu Song, and Huan Liu "Purification and Characterization of a Tachykinin-Like Peptide from Skin Secretions of the Tree Frog, Theloderma kwangsiensis," Zoological Science 30(7), 529-533, (1 July 2013). https://doi.org/10.2108/zsj.30.529
Received: 5 December 2012; Accepted: 1 January 2013; Published: 1 July 2013
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