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1 January 2014 Identification of p-Cresol as an Estrus-Specific Volatile in Buffalo Saliva: Comparative Docking Analysis of Buffalo OBP and β-Lactoglobulin with p-Cresol
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Abstract

Assessment of salivary volatile compounds adopting gas chromatography-linked mass spectrometry (GC-MS) analysis revealed the presence of a total of 11 compounds in the buffalo saliva irrespective of the stages in the reproductive cycle. p-cresol was identified as an estrus-specific volatile compound in the saliva. In addition, modeling of odorant-binding protein (OBP) and β-lactoglobulin revealed that OBP is highly stable and has strong binding affinity with p-cresol. Hydrogen bond interactions indicated that OBP is responsible for pheromone release through saliva. In contrast, β-lactoglobulin, which belongs to the same lipocalin family as OBP, possesses less affinity to p-cresol than OBP, suggesting that it is not involved in p-cresol binding and transport. Phylogenetic characterization revealed that bovine family of OBP is separately clustered. It is suggested that p-cresol has the potential to be developed as a biomarker to detect the reproductive status in the buffalo and for behavioral manipulations.

© 2014 Zoological Society of Japan
Kandasamy Karthikeyan, Paramasivan Manivannan, Durairaj Rajesh, Subramanian Muthukumar, Gangatharan Muralitharan, Mohammad Abdulkader Akbarsha, and Govindaraju Archunan "Identification of p-Cresol as an Estrus-Specific Volatile in Buffalo Saliva: Comparative Docking Analysis of Buffalo OBP and β-Lactoglobulin with p-Cresol," Zoological Science 31(1), 31-36, (1 January 2014). https://doi.org/10.2108/zsj.31.31
Received: 23 July 2013; Accepted: 16 September 2013; Published: 1 January 2014
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