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1 May 2014 Influence of Recovery Method and Centrifugation on Epididymal Sperm from Collared Peccaries (Pecari tajacu Linnaeus, 1758)
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Abstract

In order to establish protocols for gamete recovery from accidentally killed wild animals, or to take advantage of those slaughtered by captive breeders, we assess the influence of two methods on the recovery of epididymal sperm from collared peccaries, and verify the effect of centrifugation on such gametes. Genitalia from nine animals were used. For each animal, one epididymis was processed by flotation and the other was processed by retrograde flushing, both using a buffered media based on Tris. Following recovery, sperm were evaluated for motility, vigor, viability, functional membrane integrity, and morphology. A 1-mL aliquot of each sample was centrifuged, the supernatant removed, and the pellet suspended and evaluated as fresh samples. The sperm characteristics did not differ between the samples collected by flotation or retrograde flushing (P < 0.05). Centrifugation promoted an increase in head and tail defects, thus reducing the percentage of viable sperm (P < 0.05). No other parameter assessed for both methods was affected by centrifugation. In conclusion, epididymal sperm from collared peccaries can be efficiently collected through flotation or retrograde flushing, but not when either is followed by centrifugation.

© 2014 Zoological Society of Japan
José Artur Brilhante Bezerra , Andréia Maria da Silva, Gislayne Christianne Xavier Peixoto, Mariana de Araújo da Silva, Moacir Franco de Oliveira, and Alexandre Rodrigues Silva "Influence of Recovery Method and Centrifugation on Epididymal Sperm from Collared Peccaries (Pecari tajacu Linnaeus, 1758)," Zoological Science 31(5), 338-342, (1 May 2014). https://doi.org/10.2108/zs130149
Received: 9 July 2013; Accepted: 15 January 2014; Published: 1 May 2014
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