Three new species of the neogastropod family Raphitomidae Bellardi, 1875, are described from the Croatian waters of the Adriatic Sea: Raphitoma petanii Prkić, Giannuzzi-Savelli & Pusateri n. sp., R. pusaterii Prkić & Giannuzzi-Savelli n. sp. and R. stanici Prkić, Giannuzzi-Savelli & Pusateri n. sp. The new species are diagnosed by teleoconch characters and by genetic data (COI sequences) in an integrative taxonomy framework. They belong to a group of species with microgranules on the external surface of all teleoconch whorls and all three have a multispiral protoconch indicating a planktotrophic larval phase. The first two species are so far known only from material found in Croatia. R. petanii Prkić, Giannuzzi-Savelli & Pusateri n. sp. and R. stanici Prkić, Giannuzzi-Savelli & Pusateri n. sp. are predominantly shallow-water species, whereas R. pusaterii Prkić & Giannuzzi-Savelli n. sp. has been so far collected only from deeper bottoms with presence of the red coral, Corallium rubrum (Linnaeus, 1758). Syntypes of Murex echinatusBrocchi, 1814 are figured and commented.