Sertifera hirtziana Baranow & Kolan. sp. nova (Orchidaceae, Sobralieae) is described and illustrated based on Ecuadorian material. It resembles S. colombiana and S. albiflora but its erect, stiff, unequally bidentate leaves, short peduncle and lack of basal lip callus make it easily distinguishable from congenerics. Based on the inflorescence structure S. hirtziana is classified within the nominal section of Sertifera. An updated identification key to species of Sertifera the section Sertifera is provided.