Isolation and characterisation of microsatellites for the endangered Slater’s skink, Liopholis slateri (Squamata : Scincidae), via next-generation sequencing

Michael G. Gardner; Mina H. Ansari; Claire E. Treilibs; Angharad Johnston; Chris R. Pavey; C. Michael Bull

doi:10.1071/ZO17053

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20 November 2017 Isolation and characterisation of microsatellites for the endangered Slater’s skink, Liopholis slateri (Squamata : Scincidae), via next-generation sequencing

Michael G. Gardner, Mina H. Ansari, Claire E. Treilibs, Angharad Johnston, Chris R. Pavey, C. Michael Bull

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Australian J. of Zoology, 65(3):200-202 (2017). https://doi.org/10.1071/ZO17053

Abstract

We characterised 14 new polymorphic microsatellite loci for the endangered lizard Liopholis slateri. Initially, 454 shotgun sequencing was used to identify 46 loci, which were trialled for amplification. Subsequently, 14 of these loci were screened for variation in 21 individuals from scat-derived DNA samples collected from Owen Springs Reserve in central Australia. All 14 loci were polymorphic, with observed heterozygosity ranging from 0.19 to 0.86 and the number of alleles per locus ranging from 2 to 10. These loci will be useful in understanding the genetic variation and connectivity within and among extant L. slateri populations.

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Michael G. Gardner, Mina H. Ansari, Claire E. Treilibs, Angharad Johnston, Chris R. Pavey, and C. Michael Bull "Isolation and characterisation of microsatellites for the endangered Slater’s skink, Liopholis slateri (Squamata : Scincidae), via next-generation sequencing," Australian Journal of Zoology 65(3), 200-202, (20 November 2017). https://doi.org/10.1071/ZO17053

Received: 23 August 2017; Accepted: 1 October 2017; Published: 20 November 2017

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