Avian influenza is a serious threat to both animal and human health. To enable cutting-edge research in this field, we developed a molecular test on the basis of real-time polymerase chain reaction (real-time PCR), which detects influenza virus RNA. The test enables highly sensitive detection of influenza virus A and B strains, including H5N1, and specific identification of influenza A virus H5 subtypes. The kit was tested against a broad panel of influenza A and B subtypes and other respiratory viruses in collaboration with worldwide authoritative laboratories and shows a very high specificity and sensitivity. An internal control verifies RNA extraction as well as real-time PCR success. With this kit, rapid and reliable detection of influenza A and B viruses and identification of H5 subtypes can be achieved.
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1 March 2007
Sensitive and Specific Detection of Influenza Virus A Subtype H5 with Real-Time PCR
J. Rossi,
S. Cramer,
T. Laue
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Avian Diseases
Vol. 51 • No. s1
March 2007
Vol. 51 • No. s1
March 2007
avian influenza virus
H5N1
real-time RT-PCR
RRT-PCR
viral RNA extraction