We previously described the use of an established reverse genetics system for the generation of recombinant human influenza A viruses from cloned cDNAs. Here, we have assembled a set of plasmids to allow recovery of the avian H5N1 influenza virus A/Turkey/England/50-92/91 entirely from cDNA. This system enables us to introduce mutations or truncations into the cDNAs to create mutant viruses altered specifically in a chosen gene. These mutant viruses can then be used in future pathogenesis studies in chickens and in studies to understand the host range restrictions of avian influenza viruses in humans.
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1 March 2007
Development of a Reverse Genetics System Enabling theRescue of Recombinant Avian Influenza Virus A/Turkey/England/50-92/91 (H5N1)
Wendy Howard,
Anna Hayman,
Angie Lackenby,
Alison Whiteley,
Brandon Londt,
Jill Banks,
John McCauley,
Wendy Barclay
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Avian Diseases
Vol. 51 • No. s1
March 2007
Vol. 51 • No. s1
March 2007
influenza
reverse genetics