We report a new host record for Protechinostoma mucronisertulatum. These small trematode parasites were found in a debilitated, immature, male sandhill crane (Grus canadensis) during autumn migration from the Canadian prairies. Necropsy examination identified fibrinonecrotizing and ulcerative jejunitis with a focal perforation and associated local fibrinous coelomitis. Cross sections of P. mucronisertulatum were present within lesions, although their role in the pathogenesis of these lesions is undetermined. Prior reports of natural infections attributed to these flukes are rare and have been limited primarily to sora rails (Porzana carolina) from the central North American flyway. Specimens in the sandhill crane were morphologically consistent with the original description; we provide the first complete series of measurements from flukes derived from a natural infection.