Salvinia molesta D. S. Mitchell (Salviniales: Salviniaceae), a small floating fern introduced from South America, is causing an increasing number of problems in the US. Increased reliance on the biocontrol agent, Cyrtobagous salviniae, in the US is becoming more commonplace and several mass-rearing facilities have been developed. Because of differences in sampling protocols including sample size, reporting parameters, and numbers released, an investigation into sampling efficiency was initiated. A small pond in southern Louisiana was sampled in an effort to understand what constitutes an adequate sample size and methodologies needed to estimate numbers of weevils. A clumped distribution in the pond was identified, which required a large number of samples to be taken to minimize differences in means and variation. When randomly selecting 10 sets of samples where n = 5 for Sep, means varied from a high of 280 weevils/m² to a low of only 50 weevils /m², a difference of nearly 6-fold. However, when randomly selecting 10 sets of samples where n = 20, means were much more consistent and varied from a high of approximately 250 weevils/m² to a low of 125 weevils/m², a difference of only 2-fold. Sampling is expensive and to gain the most information based on the number of samples taken it is recommended that 1) the confidence interval be reported, especially when releasing weevils based on an estimation of population size; 2) understand spatial distribution and sample accordingly; and 3) when possible, initiate pilot sampling programs to acquire prior information on sampling biases, sampling errors, and differences in distribution.