Temperature and salinity and their interaction exert a major control on the life cycle of the eastern oyster (Crassostrea virginica), affecting reproduction, development, growth, and mortality. Quantifying specific temperature and salinity relationships on oyster growth and mortality has, however, proven difficult, with data suggesting potentially region-specific responses. Legacy and recent data from field tray studies from public oyster grounds in Barataria Bay (BA) and Breton Sound (BS) were used to estimate growth and mortality rates as a function of temperature and salinity. Previous studies conducted in BA and BS reported differences in growth and mortality between the basins. In the present study, environmental conditions were synchronized to compare growth and mortality between basins at similar combinations of temperature and salinity. Results indicate that when temperature and salinity are the same (synchronized), seasonal oyster growth and mortality rates still differ between BA and BS. Given the same salinity and temperature conditions, differences in growth and mortality rates between estuaries may persist because of differences in other environmental conditions (i.e., food quality and composition, hydrology, site history, and salinity variation) or localized genetic adaptations to environmental conditions.