Differentiation of Aedes atlanticus and Aedes tormentor by Restriction Fragment Length Polymorphisms of the Second Internal Transcribed Spacer

Charles B Sither; Virginia E Hopkins; Bruce A Harrison; Brittania J Bintz; E. Y Hickman; Jeffrey S Brown; Mark R Wilson; Brian D Byrd

doi:10.2987/13-6335.1

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1 December 2013 Differentiation of Aedes atlanticus and Aedes tormentor by Restriction Fragment Length Polymorphisms of the Second Internal Transcribed Spacer

Charles B Sither, Virginia E Hopkins, Bruce A Harrison, Brittania J Bintz, E. Y Hickman, Jeffrey S Brown, Mark R Wilson, Brian D Byrd

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J. of the American Mosquito Control Association, 29(4):376-379 (2013). https://doi.org/10.2987/13-6335.1

Abstract

Using novel DNA sequence data, we designed a restriction enzyme assay that distinguishes Aedes atlanticus and Ae. tormentor, based on size polymorphisms. The restriction endonuclease Hpy188I digests polymerase chain reaction–amplified 2nd internal transcribed spacer products once for Ae. atlanticus and twice for Ae. tormentor, thus providing a useful method for identifying adult female collections that are generally considered morphologically indistinguishable.

2013 by The American Mosquito Control Association, Inc.

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Charles B Sither, Virginia E Hopkins, Bruce A Harrison, Brittania J Bintz, E. Y Hickman, Jeffrey S Brown, Mark R Wilson, and Brian D Byrd "Differentiation of Aedes atlanticus and Aedes tormentor by Restriction Fragment Length Polymorphisms of the Second Internal Transcribed Spacer," Journal of the American Mosquito Control Association 29(4), 376-379, (1 December 2013). https://doi.org/10.2987/13-6335.1

Published: 1 December 2013

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