The grasshopper Sphenarium purpurascens (Charpentier) (Orthoptera: Pyrgomorphidae) is one of the most important pests of crops in Mexico. Entomopathogenic fungi used as biological control agents in different agroecosystems are natural enemies of the insect. Lethal effects of two native strains of Metarhizium anisopliae (Metchn.) Sorokin, and a commercial strain of Metarhizium acridum (Driver and Milner) were evaluated against S. purpurascens purpurascens, by measuring lethal concentrations 50 and 90 (LC₅₀ and LC₉₀). The native strain with most pathogenicity was compared with the commercial strain in the field. LC₅₀ and LC₉₀ for strain 6MaEM under controlled conditions were 1.7 × 10⁵ and 1.0 × 10⁶ conidia/ml compared to 1.7 × 10⁶ and 7.1 × 10⁶ for strain 9MaEM. Cumulative survival of M. anisopliae and M. acridum 28 days after application in the field was 53.99 and 2.06%, respectively. Sporulation of 6MaEM was 8.3%, compared to 27.63% by M. acridum. M. anisopliae had acceptable pathogenicity under field conditions, but M. acridum killed the most grasshoppers. More isolates are needed and doses and formulations need to be adjusted in future studies to have a pool of biological control options.