Four males of Amblyomma longirostre (Acari: Ixodidae) were collected on the rodent Coendou bicolor (Erethizontidae) at Área de Conservación Municipal Mishquiyacu Rumiyacu-Almendra (06°04′30.2″S, 76°58′33.5″W), Orquidiario Waqanki, Province of Moyabamba, Department of San Martín, Peru. This is the first Peruvian record of A. longirostre, which in South America has previously been reported from Argentina, Bolivia, Brazil, Colombia, French Guiana, Paraguay, Uruguay and Venezuela. Mammals and birds of various orders have been cited as hosts for adults of A. longirostre, but most records are from erethizontid rodents of the genera Coendou, Chaetomys and Sphiggurus. Birds appear to be the principal hosts for immature stages, although most records are from members of the order Passeriformes. Amblyomma longirostre appears not to be a threat to domestic vertebrates, from which it has seldom been reported, and its role as a vector of human rickettsioses is undetermined.