The ribosomal internal transcribed spacer ITS2 is probably the most popular nuclear DNA marker used to examine relationships among and within species in animals and plants. ITS2 sequences have also begun to be used as DNA barcodes. ITS2, however, has rarely been used in studies of spiders. Here, I examine the potential utility of this marker for spider phylogenetics based on preliminary data for Anelosimus spiders and a brief summary of prior work. The secondary structure of ITS2 facilitated alignment of highly divergent sequences and indicated that secondary structure morphology might be phylogenetically informative in itself. Phylogenetic analysis of Anelosimus species was congruent with a prior study based on a combination of six mitochondrial and nuclear loci plus morphology regarding the deeper clades within the genus. However, ITS2 had insufficient variation to resolve relationships within species and among closely related species. Previous studies have also discovered relatively little within-species variation in ITS2. In sum, ITS2 is an easily amplified and sequenced marker that is underutilized in spider phylogenetics; however, it has limited uses at the lowest taxonomic levels and is not likely to be a universally useful DNA barcode marker.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.