A new species of the pseudoscorpion genus SpelaeochernesMahnert, 2001, Spelaeochernes popeyesp. nov., is described from male, female and nymphal specimens collected in limestone caves of the Canudos supergroup in northeastern Brazil. It differs from the other Spelaeochernes species by the spermatheca morphology and the absence of eyespots. It exhibits marked sexual dimorphism, with an enlargement of the male pedipalp hand, which may suggest a close relationship with S. armatusMahnert, 2001 and S. dentatusMahnert, 2001. However, the spermathecal morphology suggests a relationship with S. bahiensisMahnert, 2001. Spelaeochernes popeye sp. nov. is a guanobite, and only occurs in or near frugivorous guano piles and it was only found in subterranean domain, being considered as a troglobite.