Enteromius mattozi Guimaraes, 1884, a popular eating fish among local people, were examined for monogenean parasites as part of a larger study to ascertain the feasibility of establishing aquaculture production in Limpopo, South Africa. In this study a new monogenean parasite of the genus Dactylogyrus was collected from the gills of E. mattozi, and described. It differs from known species of this group in the shape, size and structure of the heavily sclerotized vaginal prop. The proximal end of the vaginal prop has three layers similar to stacked funnels of decreasing sizes leading into a tube. The male copulatory organ is also much larger and robust than those of related species. The name Dactylogyrus mattozii is proposed for this new species.