Fern gametophytes are an often-overlooked part of field studies on ferns due to the challenge of finding material and the difficulty of identifying wild-collected plants. Most existing gametophyte descriptions are derived from lab-reared plants and while it is thought that many of the morphological features are similar under both conditions, we still have a poor understanding of in situ morphology for most taxa. The goal of this work was to document a set of morphological observations from the gametophytes of six different species collected in situ. We discovered identifiable gametophyte populations of Elaphoglossum latifolium, Hymenophyllum myriocarpoum, Lomariopsis maxonii, Polypodium dulce, Polytaenium cajenense, and Sticherus bifidus during the 2022 OTS Tropical Ferns and Lycophytes course in Costa Rica. Gametophytes were collected in the field, observed, and photographed using stereo and compound microscopy. This work represents the first detailed description for these species' gametophytes from field collections and offers insights into their natural morphology.