Altogether 137 species of polypores were identified, based on specimens collected from the Guangxi Autonomous Region, southern China. A checklist of the polypores with collection data is supplied. Three new species, Junghuhnia flabellata H.S. Yuan & Y.C. Dai, Rigidoporus fibulatus H.S. Yuan & Y.C. Dai and Trechispora suberosa H.S. Yuan & Y.C. Dai, are described and illustrated. Junghuhnia flabellata is characterized by its fiabelliform basidiocarps, small pores and small basidiospores, and skeletoystidia mostly present in dissepiments. Rigidoporus fibulatus is characterized by ceraceous to cartilaginous basidiocarps, clamp connections on generative hyphae and broadly ellipsoid to subglobose basidiospores. Trechispora suberosa is a poroid species with corky basidiocarps, ovoid to subglobose basidiospores with a finely echinulate ornamentation, and the absence of crystals on hyphae.