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18 January 2024 Germination Response of Three Smoke- and Karrikinolide-Responsive Species to Gibberellic Acid and Reactive Oxygen Species
Şükrü Serter Çatav, Kenan Akbaş, Çağatay Tavşanoğlu, Köksal Küçükakyüz
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Abstract

There is a growing interest in understanding the mechanisms involved in seed germination stimulated by smoke and smoke-derived compounds. In this study, we aimed to determine the possible role of gibberellins (GAs) and reactive oxygen species (ROS) in the seed germination of three smoke-responsive and fire-adapted species, viz. Onopordum caricum (Asteraceae), Sarcopoterium spinosum (Rosaceae), and Stachys cretica (Lamiaceae). We conducted four germination experiments using smoke water, karrikinolide (KAR1), cyanohydrin mandelonitrile, gibberellic acid (GA3), paclobutrazol, hydrogen peroxide, and several agents generating ROS at different concentrations. The three species had a positive germination response to KAR1. Sarcopoterium spinosum seeds were also sensitive to mandelonitrile. Exogenous GA3 promoted the germination of all KAR1-responsive species in a concentration-specific way. Furthermore, GA biosynthesis was found to be required for the induction of germination by smoke water and KAR1. Finally, ROS treatments stimulated the germination of KAR1- and cyanohydrin-sensitive S. spinosum seeds, but not only KAR1-sensitive O. caricum and S. cretica seeds. In conclusion, we showed that smoke-promoted germination is far more complex than previously believed and that future studies should be carried out using species with different sensitivities to smoke chemicals.

Şükrü Serter Çatav, Kenan Akbaş, Çağatay Tavşanoğlu, and Köksal Küçükakyüz "Germination Response of Three Smoke- and Karrikinolide-Responsive Species to Gibberellic Acid and Reactive Oxygen Species," Annales Botanici Fennici 61(1), 31-40, (18 January 2024). https://doi.org/10.5735/085.061.0106
Received: 7 November 2023; Accepted: 12 January 2024; Published: 18 January 2024
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