Development of microsatellite markers using 454 sequencing for the rare socially parasitic hoverfly, Microdon mutabilis

Juergen von Zum Hof; Karsten Schönrogge; James M. Cook; Michael G. Gardner

doi:10.1071/ZO12042

We can help you reset your password using the email address linked to your BioOne Complete account.

Registered users receive a variety of benefits including the ability to customize email alerts, create favorite journals list, and save searches. Please note that a BioOne web account does not automatically grant access to full-text content. An institutional or society member subscription is required to view non-Open Access content. Contact helpdesk@bioone.org with any questions.

All Fields are Required

First Name

Last/Family Name

Password

Password Requirements: Minimum 8 characters, must include as least one uppercase, one lowercase letter, and one number or permitted symbol Valid Symbols for password:
~ Tilde
! Exclamation Mark
@ At sign
$ Dollar sign
^ Caret
( Opening Parenthesis
) Closing Parenthesis
_ Underscore
. Period

Confirm Password

Web Account created successfully

Juergen von Zum Hof,¹ Karsten Schönrogge,² James M. Cook,³ Michael G. Gardner^1,*

¹ASchool of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia.
²BCentre for Ecology and Hydrology, CEH Wallingford, Maclean Building, Benson Lane, Wallingford, OX10
³CSchool of Biological Sciences, University of Reading, Reading, Berkshire, RG6 6BX, UK.

^*Corresponding author. Email: michael.gardner@flinders.edu.au

Abstract

To date there have been only limited fine-scale investigations into the molecular ecology of the European hoverfly, Microdon mutabilis, due to the paucity of available polymorphic markers. We describe the development of primers amplifying five novel microsatellite loci using next-generation sequencing (454) and three previously undescribed M. mutabilis microsatellite loci using enrichments. In hoverflies from a population in Ireland, the number of alleles per locus ranged from 2 to 16, and the observed heterozygosity ranged between 0.26 and 0.97

Citation Download Citation

Juergen von Zum Hof, Karsten Schönrogge, James M. Cook, and Michael G. Gardner "Development of microsatellite markers using 454 sequencing for the rare socially parasitic hoverfly, Microdon mutabilis," Australian Journal of Zoology 60(2), 108-110, (20 August 2012). https://doi.org/10.1071/ZO12042

Received: 18 April 2012; Accepted: 1 July 2012; Published: 20 August 2012

You currently do not have any folders to save your paper to! Create a new folder below.