The neriid fly Telostylinus angusticollis is being developed as a model organism for experimental research on developmental plasticity, nongenetic inheritance, ageing and sexual selection. No genetic information is currently available for T. angusticollis, so to facilitate paternity analyses for experimentation we characterised 17 polymorphic microsatellites for this species based on MiSeq sequences. The loci had, on average, 4.1 alleles and the observed heterozygosity ranged from 0.250 to 0.889. We show that MiSeq can be used successfully to develop microsatellite markers.
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16 May 2013
Isolation and characterisation of polymorphic microsatellite loci for the neriid fly Telostylinus angusticollis using MiSeq sequencing
Anna M. Kopps,
Russell Bonduriansky,
Anthony S. Gilchrist,
Angela J. Crean
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Australian Journal of Zoology
Vol. 60 • No. 6
May 2013
Vol. 60 • No. 6
May 2013
Neriidae
paternal effect