The range of the greater glider (Petauroides volans) is predicted to contract with climate change. Following indications of a decline in the Blue Mountains, we collated records and undertook surveys in 2015–16 to assess whether a decline has occurred and whether the decline is associated with climate change or other factors. We were unable to relocate greater gliders at 35% of our study sites, even though all were in known former locations. The species is now rare at lower elevations but remains relatively common at higher elevations: about seven times more abundant above 500 m than below. Historical data suggest that in 1986–96 it occurred in similar abundance across all elevations, 80–1060 m. Nine habitat variables accounted for 84% of the variation in greater glider density between our study sites, with significant independent contributions from elevation (37%) and time since fire (23%). We found no evidence that greater gliders have been impacted by increasing numbers of owls or cockatoos or that either the fire regime or rainfall has changed in the last 20 years. The most likely cause of the decline is the direct and indirect effects of a marked increase in temperature in the Blue Mountains. Similar declines are likely throughout the distribution of the species with increasing climate change.