The isolation and molecular characterization of pigeon paramyxovirus type 1 (PPMV-1) from a sick racing pigeon in Uruguay is reported for the first time. Hemagglutination inhibition (HI) tests were performed to detect antibodies against avian paramyxovirus serotype 1 (APMV-1), and a HI titer of 1/32 was obtained. Tracheal and cloacal swabs were processed by real-time reverse transcription–polymerase chain reaction (rRT-PCR) with the use of the National Veterinary Services Laboratory–U.S. Department of Agriculture validated matrix (M) gene assay and were positive for APMV-1. Viral isolation in embryonated chicken eggs confirmed the molecular detection of the isolate. A fragment corresponding to the 3′ region of the fusion (F) protein gene was amplified by RT-PCR, and subsequently sequenced. The deduced amino acid sequence at the F protein cleavage site displayed the motif 112RRQKR/F117. Phylogenetic analysis of this part of the genome allowed the isolated virus to be grouped in the lineage VIb/4b, which suggests that it shares the same ecologic niche with other PPMV-1 that were found in the region, and it is not imported as other European or North American viruses.
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1 March 2012
Genetic Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Columba livia in Uruguay
E. R. Castro,
F. Zanetti,
J. Arbiza
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Avian Diseases
Vol. 56 • No. 1
March 2012
Vol. 56 • No. 1
March 2012
genetic characterization
phylogenetic analysis
pigeon paramyxovirus