Aspects of the floristics and phytogeography of species of Kalanchoe Adans. (Crassulaceae subfam. Kalanchooideae) in the Waterberg, Limpopo province, northern South Africa, are discussed. In 2001 the Waterberg, which covers an area of 654,033ha (1,616,150.74 acres), was recognised as a Biosphere Reserve. It is shown that after eastern southern Africa (KwaZulu-Natal, Mpumalanga, eastern Limpopo, South Africa; Eswatini (formerly) Swaziland; southern Mozambique), the Waterberg has the most diverse Kalanchoe flora in southern Africa. A floristic synopsis of, and an identification key to, the kalanchoes of the Waterberg is presented. All the species are described and illustrated.