We previously reported that high levels (≥50%) of black soldier fly larvae meal (BSFLM) reduced growth and altered organs morphology in broiler chickens. Herein we further examined gastrointestinal, breast, bone, plasma, and litter attributes in broiler chickens fed partial to complete replacement of soybean meal (SBM) with BSFLM. A total of 1152 day-old Ross × Ross 708 male chicks (n = 8) were allocated to 48 pens and assigned one of six diets: a basal corn-SBM diet (0BSFLM), four diets in which SBM in 0BSFLM was replaced with BSFLM at 12.5%, 25%, 50%, and 100% (12.5BSFLM, 25BSFLM, 50BSFLM, and 100BSFLM) and a final diet (0 + AGP) in which 0BSFLM was fed with coccidiostat and antibiotic. Birds were bled for plasma and necropsied for samples. Litter samples were collected on days 45 to 47. Breast weight, woody breast, and hardness scores and tibia morphometry reduced linearly in response to BSFLM inclusion (P < 0.001). BSFLM linearly increased plasma Lys, Met, Thr, uric acid, creatine kinase and decreased Arg and potassium (P < 0.05). Litter ammonium nitrogen and potassium decreased linearly in response to BSFLM (P < 0.001). In conclusion, the data suggested physiological and metabolic inefficiencies in broiler chickens fed isocaloric and isonitrogenous diets with ≥50% BSFLM replacement of SBM.