Thirty-six sows were randomly assigned to three treatments and fed 0, 125, or 250 g kg^-1 corn distillers dried grains with solubles (DDGS), which aimed to evaluate the effects of feeding DDGS to sows during gestation on the fatty acid composition of colostrum and offspring. In colostrum, feeding 0–250 g kg^-1 DDGS resulted in linear increase (P < 0.001) in linoleic acid (18:2n-6) level (from 14.7% to 19.8%) and total polyunsaturated fatty acid (PUFA) level (from 16.9% to 22.8%), and linear decrease (P < 0.05) in levels of palmitic acid (16:0), oleic acid (18:1 cis-9), and total saturated fatty acid (SFA). In addition, the percentage of arachidonic acid (20:4n-6) in colostrum was increased from 0.56% to 1.23% (linear effect, P < 0.001, and quadratic effect, P = 0.010) with increasing level of DDGS. Feeding DDGS to sows resulted in linear increase (P < 0.05) in levels of 18:2n-6 (from 13.9% to 20.4%), 20:4n-6 (from 0.53% to 0.73%), docosahexaenoic acid (22:6n-3, from 0.28% to 0.37%), and total PUFA (from 15.9% to 22.6%), and linear decrease (P < 0.001) in levels of 16:0 and SFA of carcass of newborn piglets. The results demonstrated that feeding 125 or 250 g kg^-1 corn DDGS to sows increased the proportions of 18:2n-6, 20:4n-6, and total PUFA and decreased the proportions of 16:0 and total SFA in sow colostrum and the carcasses of newborn piglets, implying that inclusion of corn DDGS in diets for sows can alter fatty composition of colostrum and piglets.