Identification of Canadian barley varieties by high-throughput SNP genotyping

Sung-Jong Lee; Maria Eckhardt; Mathieu Dusabenyagasani; Marta Izydorczyk; Tigst Demeke; Daniel Perry; Sean Walkowiak

doi:10.1139/cjps-2023-0187

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Sung-Jong Lee,¹ Maria Eckhardt,¹ Mathieu Dusabenyagasani,¹ Marta Izydorczyk,^1,2 Tigst Demeke,¹ Daniel Perry,¹ Sean Walkowiakhttps://orcid.org/0000-0002-3866-5038^1,2,*

¹Canadian Grain Commission, Winnipeg, MB, Canada
²University of Manitoba, Winnipeg, MB, Canada

^*Corresponding author: Sean Walkowiak (email: sean.walkowiak@grainscanada.gc.ca)

Abstract

Barley is a major grain crop in Canada and is used for generating malt, in addition to being used as human food and animal feed. Differentiating barley varieties is important for malt quality assurance and grain handling. Here, we present a DNA-based testing method for the identification of Canadian barley varieties. The method uses 24 custom TaqMan genotyping assays, which are analyzed using the high-throughput SmartChip system (Takara Bio Inc.). Using this method, we are able to distinguish 124 barley varieties commonly grown in Canada.

Citation Download Citation

Sung-Jong Lee, Maria Eckhardt, Mathieu Dusabenyagasani, Marta Izydorczyk, Tigst Demeke, Daniel Perry, and Sean Walkowiak "Identification of Canadian barley varieties by high-throughput SNP genotyping," Canadian Journal of Plant Science 104(4), 388-393, (12 March 2024). https://doi.org/10.1139/cjps-2023-0187

Received: 1 December 2023; Accepted: 27 February 2024; Published: 12 March 2024