Current practices of flea beetle (Phyllotreta spp.) control in Brassica napus L. rely heavily on seed treatments and due to growing concerns regarding the safety of such treatments on non-target and beneficial insect populations, genetic resistance would be beneficial for a more balanced integrated pest management strategy. However, none of the registered B. napus. canola varieties exhibit measurable resistance to flea beetle injury. To this end, an evaluation of 14 winter-type B. napus breeding lines and 15 spring-type B. napus breeding lines for resistance to flea beetle feedings was conducted, as it was found that at least one line in each family exhibited noticeably reduced flea beetle damage compared to sister lines in a breeding nursery. The study revealed natural genetic variation within B. napus for flea beetle antixenosis which could be used by dedicated breeders to develop canola varieties with higher levels of flea beetle resistance. Data indicated that host plant resistance did not vary between feeding by newly emerged adult flea beetles in the fall and the overwintered adults in the spring in either winter-type or spring-type canola, as well as for adult feeding preferences. This indicates that favourable genes identified in in either habitat-type could be used interchangeably to confer resistance even though the flea beetle life cycle is different for each planting period, while either overwintered adults or newly emerged adults can be used to evaluate feeding damage.