Neotropical Plecoptera represent an interesting subject of study, for their phylogenetic, biogeographical and ecological importance. The Plecopterofauna of a large portion of the Neotropics is represented by only one genus of Perlidae, Anacroneuria Klapálek. This work provides information about the stonefly fauna of Nicaragua, a country for which few previous investigations have been undertaken for this insect group. Eight species of Anacroneuria are currently recorded, including A. curiosa Stark, A. holzenthali Stark, A. planicollis Klapálek, A. starki Fenoglio & Morisi, A. talamanca Stark, A. litura (Pictet), and the two new records: A. divisa (Navas), and A. lineata (Navas). In addition, a key for adult males is provided and the putative nymph of A. talamanca is described.