Seventy-seven of 81 (95%) Tropidurus guarani collected in Ybycui National Park, Paraguay were found to harbor 1 or more of 7 helminth species: Oochoristica bresslaui, Parapharyngodon scleratus, Piratuba digiticaudata, Physaloptera lutzi, Spauligodon oxkutzcabiensis, Strongyluris oscari, and Skrjabinelazia intermedia. All represent new host and new locality records.