We examined 822 Jollyville Plateau Salamanders, Eurycea tonkawae, from Williamson and Travis counties, Texas, U.S.A., for endoparasites. Because this species is protected, we used a minimally invasive technique to visualize and obtain encapsulated parasites. Three salamanders (0.37%) were found to be harboring metacercaria of a Clinostomum species. We identified these digeneans as belonging to C. marginatum based on morphological characteristics and supporting molecular analyses. This is the first peer-reviewed published report of any parasite from this federally threatened salamander.