The current biogeographic knowledge of Streblidae remains poor. We report a species inventory, host association, ranges, and probable relevance as vectors of pathogens in bats from a botanical garden in a region of northeastern Puebla, Mexico. Additionally, we report new streblids for Puebla including: Aspidoptera phyllostomatis, Aspidoptera delatorrei, Metelasmus pseudopterus, Megistopoda proxima, Paratrichobius longicrus, Speiseria ambigua, Strebla curvata, Strebla guajiro, Trichobius dugesii, and Trichobius joblingi. Highly diverse urban botanical gardens such as this help to maintain biodiversity where natural vegetation and domestic plants are mixed with different animal communities. Mixed vegetation provides a refuge and food for a wide range of wildlife, including bats.