Several studies have been concerned with the influences of ionizing radiation on biological systems. So, this study aimed to investigate the effect of two gamma radiation doses (0.2 and 2Gy) on two-week-old Apis mellifera workers. Honeybee workers were irradiated with the previously mentioned gamma doses. Total protein, free amino acids and DNA content (using RAPD-PCR) were estimated. The results revealed that the low dose 0.2Gy caused a significant elevation in the total protein content, and the appearance of some extra bands. Both gamma radiation doses influenced the amino acid content of A. mellifera workers. The obtained banding patterns revealed that 0.2Gy irradiated workers are more different from the unirradiated ones than are 2Gy irradiated workers. We concluded that the low dose of gamma radiation (0.2Gy) may influence the biological parameters and enhance locomotory behavior.