This study presents the first record of Ropalomeridae (Diptera, Sciomyzoidea) on dog corpses. Flies were sampled in a fragment of Cerrado (savanna formation) located in the city of Campo Grande, state of Mato Grosso do Sul, Brazil. At the study area, two medium dog corpses were set out with a Shannon and four Pitfall traps, allocated around each one. The dog corpses were obtained from the Centro de Controle de Zoonoses de Campo Grande, MS respecting all ethical procedures. The family is represented by the genera Ropalomera Wiedemann and Willistoniella Mik. We obtained seven specimens of R. femorata (Fabricius, 1805) and one specimen of W. spatulata Marques & Ale-Rocha, 2005. This is also the first record of W. spatulata for the state of Mato Grosso do Sul (MS).