Recent decades have witnessed an intensified expansion of thermophilic organisms from southern into northern Europe. Argiope bruennichi, an orb-weaver spider species, is extending its range relatively fast and gradually becoming a common species in Europe. The aim of this study was to investigate how this relatively newly-come taxon is affecting another orb-weaver spider species and whether it demonstrates features of an invasive species. Interactions were examined between this species and another dominant species with similar body and web size, Araneus quadratus. The study areas were located in two adjacent regions in northeast Poland: the warmer Mazury Lake District and the colder Suwalki Lake District. The areas differed in both population density of the studied species as well as in climatic conditions. Six study sites were selected in each region. In the Mazury Lake District, A. bruennichi was more frequent than A. quadratus; this relationship was reversed in the Suwalki Lake District. We measured the height of the web hub above the ground and the height of the plants to which webs were fixed. Web location height was chosen as an indicator of the interaction. The results indicate that A. quadratus located its webs higher than A. bruennichi, regardless of species abundance and region. A. bruennichi does not exert a significant negative impact on A. quadratus web placement. The two species clearly prefer different heights, which in turn may determine the kind of prey they catch.