Lichen communities in many National Parks are diverse and perform essential but often poorly understood ecological roles. However, lichen diversity in many National Parks is poorly characterized. Because of this, limited interpretive resources are currently available. Here we report a preliminary checklist of the lichen species found in Great Basin National Park, White Pine County, Nevada, USA, including 230 species in 84 genera, identified predominantly from recent field surveys. While our study documents an impressive diversity of lichen species in the Park, we anticipate that a significant percentage of the lichen flora remains to be discovered. Our hope is that this preliminary checklist will provide a foundation for additional lichenological research in Great Basin National Park.