The development of cultivars resistant to tomato fruit borer, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), are very limited in Pakistan and other parts of the world because of the lack of good sources of resistance for breeding. Insect-resistant cultivars would be beneficial to decrease the indiscriminate use of pesticides on tomato crops. Seven wild tomato accessions, including Solanum galapagense Darwin & Peralta, Solanum pimpinellifolium L., Solanum cheesmaniae (Riley) Fosberg, and a susceptible check (Solanum lycopersicum L.) (all Solanaceae), were evaluated for resistance to tomato fruit borer based on oviposition preference and feeding in the choice and no-choice bioassays. In the oviposition preference test, S. cheesmaniae (VI037240) and S. galapagense (VI063174) recorded the lowest mean number of eggs, 4.40 and 7.00 per plant, respectively. The highest mortality of H. armigera larvae (90%) was recorded on S. pimpinellifolium (VI030462) compared to the susceptible check (15%). The lowest pupal weight (89.67 mg) resulted from S. galapagense (VI063174), but it was not significantly different from the S. pimpinellifolium and S. cheesmaniae accessions. Among all tested accessions, S. pimpinellifolium (VI030462) was found to be highly resistant to H. armigera based on larval mortality, pupal weight, and pupation percentage. These results can pave the way to enhance the resistance in cultivated tomato varieties to control this notorious fruit borer.