Mortality and virus titer were monitored in Solenopsis invicta colony fragments to examine the impact of diet sucrose supplementation. Mortality on d 21 reached a mean of 111.8 ± 22.0 worker ants in Solenopsis invicta virus 3-treated colonies without sugar supplementation, which was significantly greater (F = 10.0; df = 3,14; P < 0.0009) than the Solenopsis invicta virus 3-infected colonies with sugar supplementation (33.5 ± 5.8), Solenopsis invicta virus 3-uninfected colonies without sugar supplementation (17.3 ± 10.7), and Solenopsis invicta virus 3-un-infected colonies with sugar supplementation (6.7 ± 2.5).