Translator Disclaimer
1 March 2013 Thermal Requirements and Generation Estimates of Trichospilus diatraeae (Hymenoptera: Eulophidae) in Sugarcane Producing Regions of Brazil
Author Affiliations +
Abstract

Knowledge of the thermal requirements of Trichospilus diatraeae (Hymenoptera: Eulophidae) is important if it is to be used successfully in to control Diatraea saccharalis (Lepidoptera: Crambidae) in sugarcane plantations. In the current study, the development of T. diatraeae was investigated in the pupae of D. saccharalis incubated at different temperatures. Seven T. diatraeae females were placed with host pupae for 24 h in tubes within chambers at 16, 19, 22, 25, 28 and 31 °C with 70 ± 10% RH and 14:10 h L:D. The life cycle duration of T. diatraeae decreased as the temperature increased, although no development was recorded at 31 °C. The number of T. diatraeae progeny per female ranged from 264.8 ± 40.7 (at 16 °C) to 385.1 ± 36.3 (at 25 °C), but no significant difference were recorded among temperature treatments (P > 0.05). The base temperature (Tb) and thermal constant (K) of T. diatraeae were 9.37 °C and 257.60 degree-days, respectively. The estimated average numbers of generations per year of T. diatraeae in pupae of D. saccharalis were 18.5, 19.93 and 17.73 for Dourados, Ivinhema and Ponta Porã municipalities of Mato Grosso do Sul State, Brasil, respectively.

The pupal parasitoid Trichospilus diatraeae Cherian & Margabandhu (Hymenoptera: Eulophidae) has been reported from species of various lepidopteran families, including Arctiidae (Zaché et al. 2012), Geometridae (Pereira et al. 2008; Zaché et al. 2010; Pastori et al. 2012), Nymphalidae (Bouˆek 1976), Noctuidae (Paron & Berti-Filho 2000), Oecophoridae (Oliveira et al. 2005), Pieridae (Kazmi & Chauhan 2003), Pyralidae (Bennett et al. 1987; Melo et al. 2011) and Riodinidae (Zaché et al. 2011). This natural enemy can also parasitize and develop in pupae of Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) (Paron & Berti-Filho 2000), indicating its potential for use in the biological control of this insect borer of sugar cane.

Diatraea saccharalis is the most important pest of sugarcane in Brazil (Dinardo-Miranda et al. 2012). Feeding injury to the stem results in direct damage, where as indirect losses occur because of microorganism growth that results in decrease of sugar and alcohol production (Pinto et al. 2009). Chemical insecticides have low efficiency against D. saccharalis because its larvae develop in galleries within the sugarcane stem. This increases the importance of using parasitoids for biological control of this pest. In Brazil, the larval endoparasitoid Cotesia flavipes Cameron (Hymenoptera: Braconidae) is the most efficient means of controlling D. saccharalis (Silva et al. 2012), but the egg parasitoid Trichogramma galloi Zucchi (Hymenoptera: Trichogrammatidae) also shows promise in controlling pest populations (Parra & Zucchi 1997).

Diatraea saccharalis is attacked by T. diatraeae (La Salle 1994). However, little is known about this parasitoid, including its temperature range. The relation between environmental temperature and insect development can be used to determine the best conditions to rear insects that will be released in the field and to predict the number of generations of the pest and its natural enemies (Nava et al. 2007; Wanderley et al. 2007; Pereira et al. 2011).Temperatures above or below an optimal value can affect insect development (Rodrigues et al. 2004). Determining the optimum temperature can allow production of a large number of individuals with adequate survival, and with high reproductive and parasitism rates, both in laboratory and in the field (Medeiros et al. 2004; Pereira et al. 2004; Pratissoli et al. 2006; Zago et al. 2006; Pasini et al. 2010). The ability of natural enemies to survive at different temperatures can also increase their impact in biological control programs (Pratissoli et al. 2005; Iranipour et al. 2010).

The objective of the current study was to determine the thermal requirements of T. diatraeae in D. saccharalis pupae in the laboratory and to estimate the number of generations of this parasitoid in three municipalities that are major sugarcane producers in Mato Grosso do Sul State, Brasil.

MATERIALS AND METHODS

The experiment was conducted at the Laboratory of Entomology/Biological Control (LECOBIOL) at the Faculdade de Ciências Agrárias (FCA) of the Universidade Federal da Grande Dourados (UFGD) in Dourados, Mato Grosso do Sul, Brasil.

Rearing of Diatraea saccharalis

Eggs of D. saccharalis were obtained from the company Biosoluções. Newly hatched first-instar larvae were placed in screened vials with an artificial diet until pupa stage was reached. Pupae were then collected, sexed and groups of 50 pupae (20 males and 30 females) were placed in PVC cages (10 cm diam × 22 cm ht). Each cage was wrapped with moistened bond paper and contained a Petri dish lined with filter paper as an oviposition substrate. Each PVC cage was closed with bond paper and rubber bands. Emerging adults were fed with a 10% water and honey solution supplied to the insects through a cotton wick inserted into plastic containers (3 cm diam × 4 cm ht), modified according to Parra (2001).

Rearing of Trichospilus diatraeae

Adults of T. diatraeae were obtained from a rearing laboratory at LECOBIOL and kept in glass tubes (2.5 cm diam × 8.5 cm ht) sealed with cotton wool. The insects were fed with droplets of honey. Twenty-four-to 48-h-old D. saccharalis pupae were exposed to the parasitoid for a period of 72 h. Parasitized pupae were isolated and maintained at 25 ± 1 °C, 70 ± 10% RH and 14:10 h L:D until adult emergence (Pereira et al. 2008).

Experimental Design

Single 24-h-old D. saccharalis pupae each weighing 0.202 g were placed in glass tubes (2.5 cm diam × 14 cm ht) and exposed to seven 24- or 48-h-old T. diatraeae females for 24h. The parasitoids were then removed and the host pupae transferred to climatic chambers at 16, 19, 22, 25, 28 or 31 °C with 70 ± 10% RH and 14:10 h L:D.

The duration of the preimaginal development, the number and percent of emerged adults, and the number and sex ratio of the offspring [SR= number of females/(number of females + number of males] were evaluated. In addition, 15 T. diatraeae females were selected per treatment to evaluate their longevity. Each female was placed individually in a glass tubes (2.5 cm diam × 8.5 cm ht) with a droplet of honey. Each tube was closed with cotton and the longevity of the parasitoid recorded. The sex of each adult parasitoid was determined based on morphological characteristics of the antenna and the abdomen (Delvare & Lasalle 1993).

The experimental design was completely randomized with 6 treatments (temperatures) and 12 replications. Percentages of T. diatraeae emergence were analyzed through a generalized linear model with binomial distribution (P ≤ 0.05) with the R Statistical System (Ihaka & Gentleman 1996). Analysis of variance was conducted on the other variables and, if significant at 5% probability, to regression analysis. The equation was selected using the linear and cubic models, based on the determination coefficient (R2), significance of the regression coefficients (βi) and regression by the F-test (up to 5% probability).

The base temperature (Tb) and thermal constant (K) were calculated according to the hyperbole method (Haddad et al. 1999) using the Mobae program based on the duration of the cycle (egg-adult) of T. diatraeae at each temperature tested. The number of annual generations of this parasitoid was estimated for the municipalities of Dourados, Ivinhema, and Ponta Porã, Mato Grosso do Sul State, Brasil, with the equation: NG= {T (Tm-Tb)/K}, where K= thermal constant, Tm = average temperature for each location studied, Tb = base temperature and T = number of days per month. This was based on the normal thermal conditions of sugar cane production. Climatic data (annual temperature and relative humidity averages between 1999 and 2009) at these locations were provided by the National Institute of Meteorology (INMET).

RESULTS

The duration of the egg to adult cycle of T. diatraeae in D. saccharalis pupae decreased as the temperature increased between 16 and 28 °C (R2 = 0.79; F = 174.0; P = 0.0001; dferror = 47) (Fig. 1A). The parasitoid did not complete its development at 31 °C, therefore, its biological characteristics were not evaluated at this temperature.

Temperatures between 16 and 28 °C variously affected the emergence of T. diatraeae from D. saccharalis pupae. The percentage of D. saccharalis pupae from which the parasitoid emerged ranged from 100 to 0%, with lower values at 22, 28 and 31 °C, and higher values at 16, 19 and 25 °C (Fig. 1B). The average number of T. diatraeae progeny varied from 264.8 ± 40.7 (16 °C) to 385.1 ± 36.3 (25 °C), with no significant difference in values (P > 0.05) at these temperatures.

The longevity of T. diatraeae females was highest at 16 °C (34.4 ± 1.5 d) and lowest at 28 °C (1.5 ± 0.19 d) (R2 = 0.883; F = 178.37; P = 0.0001; dferror = 74) (Fig. 2A). The sex ratio of T. diatraeae offspring varied from 0.80 to 0.90 at 16–28 °C (P > 0.05). The thermal requirements for the immature stage of this parasitoid in D. saccharalis pupae was based on the model Y = (1/D) = -0.036393 + 0.003882x (R2 = 95.64). The values of the base temperature (Tb) and thermal constant (K) were 9.37 °C and 257.60 degree-days (DD), respectively (Fig. 2B).

The average numbers of T. diatraeae generations per year in D. saccharalis pupae can be estimated based on the mean temperatures from 1999 to 2009 for Dourados municipality, Mato Grosso do Sul, Brasil. Climate conditions of Dourados municipality might allow 18.5 generations of this parasitoid per year, whereas this number is 5.4 for D. saccharalis (Fig. 3A).The numbers of generations per year of T. diatraeae were 19.93 and 17.3 for Ivinhema and Ponta Porã, Mato Grosso do Sul, Brasil, respectively (Figs. 3B and C).

Fig. 1.

Duration of egg to adult (days) (F = 174.0391; P = 0.0001) of Trichospilus diatraeae (Hymenoptera: Eulophidae) (A) and percentage of Diatraea saccharalis (Lepidoptera: Crambidae) pupae (B) from which of T. diatraeae emerged at different temperatures and at 70 ± 10% RH and 14:10 h L:D.

f01_154.jpg

DISCUSSION

Trichospilus diatraeae developed in D. saccharalis pupae under temperature conditions of 16–28 °C. However, at 31 °C, 91.7% of host pupae contained dead pre-pupae of the parasitoid, which indicates that the upper thermal limit for this stage of this parasitoid is above 28 °C and below 31 °C. The population of T. diatraeae used in our experiments was from Viçosa, Minas Gerais State, Brasil, which has an average annual temperature of 19.4 °C. This population may not be adapted to high temperatures which might have contributed to the fact that the parasitoid was unable to complete its life cycle at 31 °C (Pereira et al. 2008). High temperatures negatively affect the early stages of parasitoids and their development can be lower or totally unviable outside the ideal temperature range (Krugner et al. 2007). However, laboratory conditions might under estimate the effects of temperature variations on different stages of parasitoids compared with field conditions (Haghani et al. 2007; Krugner et al. 2007). Therefore, T. diatraeae might complete its development in the field even at temperatures above 30 °C as long as these do not coincide with crucial development stages of this parasitoid.

Fig. 2.

Longevity (F = 178.3684; P = 0.0001) in days (A) and development speed (B) of Trichospilus diatraeae (Hymenoptera: Eulophidae) females that emerged from Diatraea saccharalis (Lepidoptera: Crambidae) pupae at different temperatures and at 70 ± 10% RH and 14:10 h L:D.

f02_154.jpg

The mean longevity of T. diatraeae was 18 days at 22–25 °C, but there was no emergence of females of this parasitoid at 31 °C from D. saccharalis, although they were able to survive for 34 days at this temperature. The life cycle of the parasitoid can increase at 16 °C in the laboratory to decrease the number of generations during lower demand for parasitoid or absence of the host. This could be used to synchronize parasitoid emergence with the presence of its host at the right stage in the field to improve the efficiency of the mass rearing and release of this natural enemy in biological control programs (Sagarra et al. 2000; Krugner et al. 2007; Bueno et al. 2008). Thermal requirements allow calculation of the time needed to complete the development of both a pest and its natural enemies (Zanuncio et al. 2004; Pereira et al. 2011). The thermal requirements of T. diatraeae in the laboratory were similar to those of D. saccharalis (Tb = 8.0 °C and K = 371.88 DD) (Paron & Berti-Filho 2000). However, this parasitoid showed a shorter development cycle compared with D. saccharalis and, therefore, a higher number of generations. This might help reduce or stabilize the population density of D. saccharalis at a low level in the field (Pastori et al. 2008; Pereira et al. 2011).

Fig. 3.

Estimated average number of generations per month of Trichospilus diatraeae (Hymenoptera: Eulophidae) in Diatraea saccharalis (Lepidoptera: Crambidae) in sugar cane producing municipalities in Mato Grosso do Sul State, Brasil (A, B and C).

f03_154.jpg

The development of T. diatraeae in D. saccharalis pupae at temperatures between 16 and 28 ° C indicates that releases of parasitoid adults to control D. saccharalis must be conducted during the morning or late afternoon. In addition, temperatures should be below 31 °C because this parasitoid population might not provide adequate levels of control at high temperatures. Parasitism alone might not be sufficient to adequately control D. saccharalis in the field. However, the overlapping of T. diatraeae generations may reduce D. saccharalis infestations in the field. The data obtained by this current study might facilitate the biological control of D. saccharalis with T. diatraeae in sugarcane producing regions, especially in Mato Grosso do Sul State, Brasil.

CONCLUSION

Trichospilus diatraeae develops in the laboratory between 16 and 28 °C in D. saccharalis pupae. The estimated number of generations of T. diatraeae per yr is higher than that of D. saccharalis, which might contribute to the successful biological control of this pest using this parasitoid species.

ACKNOWLEDGMENTS

To the Brasilian Institutions “Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and Instituto Nacional de Meteorologia (INMET)”. To the undergraduate Gabriela Piñeyro for translating the abstract to Spanish language. Asia Science corrected and edited the English of this manuscript.

REFERENCES CITED

  1. F. D. Bennett , H. Glenn , M. Yaseen , and R. M. Baranowski 1987. Records of Trichospilus diatraeae, an Asian parasite (Hymenoptera: Eulophidae) from the Caribbean and Florida. Florida Entomol. 70: 184–186. Google Scholar

  2. Z. Bouˆek 1976. The African and Asiatic species of Trichospilus and Cotterellia (Hymenoptera: Eulophidae). Bull. Entomol. Res. 65: 669–681. Google Scholar

  3. R. C. O. F. Bueno , T. R. Carneiro , D. Pratissoli , A. F. Bueno , and O. A. Fernandes 2008. Biology and thermal requirements of Telenomus remus reared on fall armyworm Spodoptera frugiperda eggs. Cienc. Rural 38: 01–06. Google Scholar

  4. E. G. Delvare , and J. Lasalle 1993. A new genus of Tetrastichinae (Hymenoptera: Eulophidae) from the Neotropical region, with the description of a new species parasitica on key pests of oil palm. J. Nat. Hist. 27: 435–444. Google Scholar

  5. L. L. Dinardo-Miranda , I. A. Anjos , V. P. Costa , and J. V. Fracasso 2012. Resistance of sugarcane cultivars to Diatraea saccharalis. Pesqui. Agropecu. Brasileira 47: 1–7. Google Scholar

  6. M. L. Haddad , J. R. P. Parra , and R. C. B. Moraes 1999. Métodos para estimar Piracicaba: os limites térmicos inferior e superior de desenvolvimento de insetos. Fealq, Piracicaba, Brasil. 29 pp. Google Scholar

  7. M. Haghani , Y. Fathipour , A. A. Talebi , and V. Baniameri 2007. Temperature dependent development of Diglyphus isaea (Hymenoptera: Eulophidae) on Liriomyza sativae (Diptera: Agromyzidae) on cucumber. J. Pest. Sci. 80: 71–77. Google Scholar

  8. R. Ihaka , and R. A. Gentleman 1996. A language for data analysis and graphics. J. Comput. Graph. Stat. 5: 299–314. Google Scholar

  9. S. Iranipour , Z. N. Bonab , and J. P. Michaud 2010. Thermal requirements of Trissolcus grandis (Hymenoptera: Scelionidae), an egg parasitoid of sunn pest. European J. Entomol. 107: 47–53. Google Scholar

  10. S. I. Kazmi , and N. Chauhan 2003. Chalcidoid parasitoids (Hymenoptera: Chalcidoidea) of Hypsipyla robusta (Lepidoptera: Pyralidae), a pest of cedars and mahogany. Oriental Insects 37: 261–275. Google Scholar

  11. R. Krugner , K. M. Daane , A. B. Lawson , and G. Y. Yokota 2007. Temperature-dependent development of Macrocentrus iridescens (Hymenoptera: Braconidae) as a parasitoid of the oblique banded leaf roller (Lepidoptera: Tortricidae): Implications for field synchrony of parasitoid and host. Biol. Control 42: 110–118. Google Scholar

  12. J. Lasalle 1994. North American genera of Tetrastichinae (Hymenoptera: Eulophidae). J. Nat. Hist. 28: 109–236 Google Scholar

  13. R. S. Medeiros , F. S. Ramalho , J. E. Serrão , and J. C. Zanuncio 2004. Estimative of Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae) development time with non linear models. Neotrop. Entomol. 33: 141–148. Google Scholar

  14. R. L. Melo , D. Pratissoli , R. A. Polanczyk , M. Tavares , A. Milanez , and D. F. Melo 2011. Ocorrência de Trichospilus diatraeae (Hymenoptera: Eulophidae) em broca-das-cucurbitáceas, no Brasil. Hortic. Brasileira 29: 228–230. Google Scholar

  15. D. E. Nava , A. M. Nascimento , C. P. Stein , M. L. Haddad , J. M. S. Bento , and J. R. P. Parra 2007. Biology, thermal requirements, and estimation of the number of generations of Zaprionus indianus (Diptera: Drosophilidae) for the main fig producing regions of Brazil. Florida Entomol. 90: 495–501. Google Scholar

  16. H. N. Oliveira , J. C. Zanuncio , E. P. Pedruzzi , and M. C. Espíndula 2005. Rearing of Thyrinteina arnobia (Lepidoptera: Geometridae) on guava and eucalyptus in the laboratory. Brazilian Arch. Biol. Techn. 48: 801–806. Google Scholar

  17. M. R. Paron , and E. Berti-Filho 2000. Capacidade reprodutiva de Trichospilus diatraeae (Hymenoptera: Eulophidae) em pupas de diferentes hospedeiros (Lepidoptera). Sci. Agr. 57: 355–358. Google Scholar

  18. J. R. P. Parra , and R. A. Zucchi 1997. Trichogramma e o controle biológico aplicado, pp. 41–66 In R. A. Zucchi , and R. C. Monteiro [eds.], O gênero Trichogramma na América do Sul. Fealq, Piracicaba, Brasil. Google Scholar

  19. J. R. P. Parra 2001. Técnicas de criação de insetos para programas de controle biologico. Fealq. Piracicaba, Brasil. 134 pp. Google Scholar

  20. A. Pasini , J. R. P. Parra , D. E. Nava , and A. R. Butnariu 2010. Exigências térmicas de Dora lineare Eschs e Doru luteipes Scudder em laboratório. Cienc. Rural. 40: 1562–1568. Google Scholar

  21. P. L. Pastori , L. B. Monteiro , and M. Botton 2008. Biologia e exigências térmicas de Trichogramma pretiosum Riley (Hymenoptera, Trichogrammatidae) linhagem bonagota criado em ovos de Bonagota salubricola (Meyrick) (Lepidoptera, Tortricidae). Rev. Brasileira Entomol. 52: 472–476. Google Scholar

  22. P. L. Pastori , F. F. Pereira , G. S. Andrade , R. O. Silva , J. C. Zanuncio , and A. I. A. Pereira 2012. Reproduction of Trichospilus diatraeae (Hymenoptera: Eulophidae) in pupae of two lepidopterans defoliators of eucalypt. Rev. Colombiana Entomol. 38: 91–93. Google Scholar

  23. F. F. Pereira , R. Barros , D. Pratissoli , and J. R. P. Parra 2004. Biologia e exigências térmicas de Trichogramma pretiosum Riley e Trichogramma exiguum Pinto & Planter (Hymenoptera: Trichogrammatidae) criados em ovos de Plutella xylostella (L.) (Lepidoptera: Plutellidae). Neotrop. Entomol. 33: 231–236. Google Scholar

  24. F. F. Pereira , J. C. Zanuncio , M. T. Tavares , P. L. Pastori , and G. C. Jacques 2008. Record of Trichospilus diatraeae (Hymenoptera: Eulophidae) as parasitoid of the eucalyptus defoliator Thyrinteina arnobia (Lepidoptera: Geometridae) in Brasil. Phytoparasitica. 36: 304–306. Google Scholar

  25. F. F. Pereira , J. C. Zanuncio , H. N. Oliveira , E. V. Grance , P. L. Pastori , and M. D. Gava-Oliveira 2011. Thermal requirements and estimate number of generations of Palmistichus elaeisis (Hymenoptera: Eulophidae) in different Eucalyptus plantations regions. Brazilian J. Biol. 71: 431–436. Google Scholar

  26. A. S. Pinto , P. S. M. Botelho , and H. N. De. Oliveira 2009. Guia ilustrado de pragas e insetos benéficos da cana-de-açúcar. CP2, Piracicaba, Brasil. 160 pp. Google Scholar

  27. D. Pratissoli , J. C. Zanuncio , U. R. Vianna , J. S. Andrade , T. B. M. Pinon , and G. S. Andrade 2005. Thermal requirements of Trichogramma pretiosum and T. acacioi (Hym.: Trichogrammatidae), parasitoids of the avocado defoliator Nipteria panacea (Lep.: Geometridae), in eggs of two alternative hosts. Brazilian Arch. Biol. Techn. 48: 523–529. Google Scholar

  28. D. Pratissoli , E. F. Reis , H. B. Zago , P. L. Pastori , and T. Tamanhoni 2006. Biologia e exigências térmicas de cinco linhagens de Trichogramma pretiosum Riley (Hymenoptera: Trichogrammatidae) criadas em ovos de Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Cienc. Rural 36: 1671–1677. Google Scholar

  29. S. M. M. Rodrigues , V. H. P. Bueno , M. V. Sampaio , and M. D. Soglia 2004. Influência da temperatura no desenvolvimento e parasitismo de Lysiphebus testaceipes (Cresson) (Hymenoptera: Braconidae, Aphidiinae) em Aphis gossypii Glover (Hemiptera: Aphididae). Neotrop. Entomol. 33: 341–346. Google Scholar

  30. L. A. Sagarra , C. Vincent , N. F. Peters , and R. K. Stewart 2000. Effect of host density, temperature, and photoperiod on the fitness of Anagyrus kamali, a parasitoid of the hibiscus mealybug Maconellicoccus hirsutus. Entomol. Exp. Appl. 96: 141–147. Google Scholar

  31. C. C. M. Silva , E. J. Marques , J. V. Oliveira , and E. C. N. Valente 2012. Preference of the parasitoid Cotesia flavipes (Cam.) (Hymenoptera: Braconidae) for Diatraea (Lepidoptera: Crambidae). Acta. Sci. Agron. 34: 23–27. Google Scholar

  32. P. A. Wanderley , F. S. Ramalho , J. C. Zanuncio , and G. L. D. Leite 2007. Thermal requirements of Bracon vulgaris a parasitoid of the cotton boll weevil. Phytoparasitica 35: 336–345. Google Scholar

  33. B. Zaché , C. F. Wilcken , R. R. Da Costa , and E. P. Soliman 2010. Trichospilus diatraeae Cherian & Margabandhu, 1942 (Hymenoptera: Eulophidae), a new parasitoid of Melanolophia consimilaria (Lepidoptera: Geometridae). Phytoparasitica 38: 355–357. Google Scholar

  34. B. Zaché , R. R. C. Zaché , E. P. Soliman , and C. F. Wilcken 2011. Evaluation of Trichospilus diatraeae (Hymenoptera: Eulophidae) as parasitoid of the eucalyptus defoliator Euselasia eucerus (Lepidoptera: Riodinidae). Intl. J. Trop. Insect Sci. 31: 118– 121. Google Scholar

  35. B. Zaché , R. R. C. Zaché , N. M. Souza , M. H. F. A. D. Pogetto , and C. F. Wilcken 2012. Evaluation of Trichospilus diatraeae (Hymenoptera: Eulophidae) as parasitoid of the eucalyptus defoliator Eupseudosoma aberrans Schaus, 1905 (Lepidoptera: Arctiidae). Biocontrol. Sci. Technol. 22: 363–366. Google Scholar

  36. H. B. Zago , D. Pratissoli , R. Barros , and M. G. C. Gondim Jr 2006. Biologia e exigências térmicas de Trichogramma pratissolii Querino & Zucchi (Hymenoptera: Trichogrammatidae) em hospedeiros alternatives. Neotrop. Entomol. 35: 377–381. Google Scholar

  37. J. C. Zanuncio , C. H. O. Silva , L Cruz , and D. Pratissoli 2004. Functional and numerical responses and reproduction of Campoletis flavicincta (Hym. :Ichneumonidae) with different densities of larvae of Spodoptera frugiperda (Lep.: Noctuidae). Biocontrol. Sci. Technol. 43: 122–127. Google Scholar

Maria Adriana Torqueti Rodrigues, Fabricio Fagundes Pereira, Samir Oliveira Kassab, Patrik Luiz Pastori, Daniele Fabiana Glaeser, Harley Nonato De Oliveira, and José Cola Zanuncio "Thermal Requirements and Generation Estimates of Trichospilus diatraeae (Hymenoptera: Eulophidae) in Sugarcane Producing Regions of Brazil," Florida Entomologist 96(1), (1 March 2013). https://doi.org/10.1653/024.096.0120
Published: 1 March 2013
JOURNAL ARTICLE
6 PAGES


SHARE
ARTICLE IMPACT
Back to Top