Translator Disclaimer
1 December 2013 Demographic Parameters of Tetranychus urticae (Acari: Tetranychidae) on Four Rosa sp. Cultivars
Author Affiliations +
Abstract

The goal of this work was to determine the life parameters of Tetranychus urticae Koch on leaves of 4 rose (Rosa sp.) cultivars. To conduct this experiment a colony of T. urticae collected from ornamentals grown at Saltillo, Coahuila, Mexico, was established on bean (Phaseouls vulgaris L.) seedlings inside a Biotronette chamber at 25 ± 2 °C, 60-70 RH and 12:12 h L:D. According to the experimental design, 100 one-day old recently mated and fertilized females were transferred to 2.5 cm diam rose (Rosa sp. L.) leaf discs from ‘Emma', ‘Luna', ‘Gran Gala’ and ‘Virginia' cultivars in such a way that every experimental unit included 1 female per disc. The latter were maintained at the above temperature, RH and photoperiod conditions. Demographic parameters in this experiment showed greater growth potential of this pest on the ‘Luna' and ‘Gran Gala’ cultivars than on ‘Virginia’ and ‘Emma’.

The two-spotted spider mite Tetranychus urticae Koch (Trombidiformes: Tetranychidae) is the main pest of greenhouse roses (Rosa sp. L.; Rosales: Rosaceae) (Van de Vrie 1985), a highly significant crop in Mexico. The detrimental effects of this pest include significant reductions in photosynthesis, stomatal conductance, transpiration and chlorophyll content (Fikru & Higley 2003; Jeppson et al. 1975). Population densities of 10 to 50 mites per leaf cause a 6 to 10% reduction in length of flower buds, compared with the control (Landeros et al. 2004). At present T. urticae on ornamental crops is primarily controlled by chemicals (Takematsu et al. 1994), but such control is becoming progressively more difficult because of rapidly developing miticide-resistance (Stumpf & Nauen 2002). Tetranychus urticae resistance to pesticides has been globally demonstrated with over 200 reported cases (Konanz & Nauen 2004). A tool for the control of pests is the use of resistant cultivars, and this tool is being used effectively in protecting many crop species (Flexner et al. 1995). Pest-resistant cultivars are a good way to improve production, minimize plant damage, improve crop quality, apply less pesticides, and decrease costs (Bustamante & Patiño 2001). Plant resistance to pests can be caused by antix- enosis, antibiosis, tolerance or some combinations of these mechanisms (Smith 2005). Antibiosis has a direct influence on the life history of a pest, and thus comparison of biological parameters of a pest species reproducing and developing on different plants of a given species can be used to select resistant varieties to a pest (Li et al. 2004). The goal of this study was to assess the demographic parameters of the two-spotted spider mite, T. urticae, on 4 rose cultivars, i:e., ‘Gran Gala’, ‘Luna’, ‘Virginia’ and ‘Emma’ in order to determine the extent to which each of these cultivars fosters the development of this pest.

Materials and Methods

Specimens of T. urticae were collected from ornamentals grown at Saltillo Coahuila, Mexico to establish a mother colony (stock colony) on bean (Phaseolus vulgaris L.; Fabales: Fabaceae) leaves inside a Biotronette environmental chamber at 25 ± 2 °C, 60-70 RH and 12:12 h L:D.

The Abbott-Setta & Childers (1987) technique was used to handle the biological material. This technique is also known as the arena-leaf technique. Thus female mites—after they had been collected by a suction tube from leaves of each rose cultivar—were transferred to circular leaf discs (2.5 cm diam) using a 000 camel's hair brush. The discs were placed up-side down on plastic trays lined with water-saturated cotton.

In order to determine the demographic parameters of T. urticae, 25 females were placed for egg laying on such leaf discs of the 4 cultivars during 24 h, then the females were separated, leaving the eggs behind. Eggs deposited on these discs were held in environmental chambers. After these eggs had hatched and the immature progeny had emerged as adults, 100 of the 1-day-old adult females, recently mated, were individually placed on a leaf disc of each rose cultivar. These mated females were kept under the same environmental conditions as the mother colony, and 1 female per disc was an experimental unit. Eggs deposited by these females were maintained on the same leaf disc until emergence of the larvae, which were then placed singly on another in leaf disc. From this point on daily survival and ovi- position of adult females were recorded until the last female died. Demographic calculations based on Birch's model (1948) were made and the Jack- nife method was used to estimate standard deviations with a confidence interval of 95%.

Results and Discussion

Survival and Fertility

Survival rates of T. urticae on the 4 rose cultivars (Fig. 1) were not significantly different from each other (log-rank test P ≤ 0.05), and the range of live females in the 4 treatments ended in a similar way. However at 13–19 days of age, a larger fraction of T. urticae females remained alive on the ‘Virginia’ discs.

The fertility rate per specific age of T. urticae on ‘Virginia’ (Fig. 2) tended to be the lowest by far, followed by that on ‘Emma’, while those on ‘Gran Gala’ and especially ‘Luna’ produced much larger numbers of female offspring. According to Tukey's test, the differences were significant between ‘Virginia’ and the other 3 cultivars in terms numbers of daughters per mother.

Fig. 1.

Survivorship curves for Tetranychus urticae Koch on four rose (Rosa sp.) cultivars, i:e., ‘Gran Gala’, ‘Luna’, ‘Virginia’ and ‘Emma’.

f01_1508.jpg

Demographic Parameters

Regarding the gross reproductive rate (GRR) or total number of T. urticae females produced per mother at all ages, the highest value (78.33) was recorded on ‘Luna’ followed by those on ‘Gran Gala’ (45.43),’ Emma’ (18.64) and ‘Virginia’ (10.44) (Table 1). The GRR values of T. urticae obtained from the present research work clearly indicate differences in reproductive performance of T. urticae on the 4 rose cultivars, but these values were lower than that reported by Maggi & Leight (1983) with a GRR of 91.26 on cotton (Gossypium hirsutum L.; Malvales: Malvaceae) leaves. On the other hand, Flores et al. (2000) reported a GRR of 218.22 on bean leaf discs, while Sáenz de Cabezón et al. (2006) reported a GRR of 85.88 for T. urticae also on bean leaf discs.

Fig. 2.

Age-specific fecundity of Tetranychus urticae Koch on four rose (Rosa sp.) cultivars, i:e., ‘Gran Gala’, ‘Luna’, ‘Virginia’ and ‘Emma’.

f02_1508.jpg

With regards to the net reproduction rate (Ro) (average number of daughters that a female produces during her lifetime), the largest number of T. urticae daughters/mother in one generation were registered on ‘Luna’ (33.46), followed by ‘Gran Gala’ (22.57), ‘Emma’ (8.78) and ‘Virginia’ (7.60). These values represent reductions in Ro of T. urticae of 32.54, 73.75 and 77.26% on these 3 cultivars, respectively, versus the ‘Luna’ cultivar (Table 1). Thus, ‘Emma’ and ‘Virginia’ allowed much slower T. urticae population development than ‘Luna’ and ‘Gran Gala’; and these results show that ‘Luna’ is the most susceptible cultivar. Also for T. urticae Marcic (2007) reported a Ro value of 28.92 on bean leaf discs, Grissa-Lebdi et al. (2002) registered a Ro value of 19.2 on apple (Malus domestica Borkh.; Rosales: Rosaceae) leaf discs and Bounfour & Tanigoshi (2001) found a Ro value of 54.86 at 25 °C on raspberry (Rubus idaeus L.; Rosales: Rosaceae) leaves.

Table 1.

Population parameters of Tetranychus urticae on leaf discs of four rose cultivars: ‘Luna’, ‘Gran Gala’, ‘Virginia’ and ‘Emma’.

t01_1508.gif

Values of the reproductive capacity of the T. urticae population (intrinsic growth rate, rm), i.e., rate at which the population increases in the absence of density-dependent forces (Table 1) indicated that ‘Gran Gala’ (0.272) allowed the greatest population increase rate of T. urticae and is most susceptible to this pest, followed by the intrinsic growth rate on ‘Luna’, (0.2550), ‘Emma’ (0.2338) and ‘Virginia’ (0.2160). Even though there is no statistical difference between these values (Tukey's P = 0.05), these values represent a reduction in the multiplying capacity of T. urticae by 6.32, 14.11 and 20.65% on these respective cultivars in comparison to ‘Gran Gala’. These results coincide with values ranging from 0.220 to 0.340 commonly reported by Sabelis (1991). Skorupska (1998) reported the rm values of 2 Tetranychus species on 5 apple cultivars, which ranged from 0.084 to 0.113, while Kheradpir et al. (2007) reported rm values of T. urticae on 5 different cultivars of Cucumis sativus L. (Cucurbitales: Cucurbitaceae) ranging from 0.254 to 0.313. These authors stated that different rm values depended mainly on the host and the temperature. This last factor is important; Bountour & Tanigoshi (2001) recorded rm values on T. urticae rm values of 0.084 at 15 °C, and 0.321 at 30 °C.

Regarding the mean time between generations (TG), a higher value of 13.72 days—with a daily population increase of 1.29—was recorded for ‘Luna’, followed by progressively shorter times on ‘Gran Gala’, ‘Virginia’ and ‘Emma’ (Table 1). These results imply a greater damage potential in ‘Luna’ cultivar, as compared with the other 3 cultivars. These results are lower than to those reported by Grissa-Labdi et al. (2002) on apple leaves. These workers recorded a TG of 19.7 days and a daily growth factor of 1.16, whereas Wermelinger et al. (1991) recorded a generation time of 15.4 days. Lastly, the doubling times (T2) of T. urticae population in ‘Virginia’, ‘Emma’, ‘Luna’ and ‘Gran Gala’ were 3.20, 2.29, 2.70 and 2.54 days, respectively (Table 1). These results represent reductions of 28.4, 15.6 and 20.6 % in time needed by T urticae develop on ‘Emma’, ‘Luna’, and ‘Gran Gala’, as compared with ‘Virginia’. ‘Virginia’ presented greatest resistance to the demographic development of T. urticae, followed by ‘Emma’, ‘Gran Gala’ and ‘Luna’.

Endnote

Yisa M Ochoa Fuentes is a Professor in the Department of Parasitology, Autonomous Agricultural University Antonio Narro, Saltillo, Coahui- la, México, i.e., Departamento de Parasitología Agrícola, Universidad Autónoma Agraria Antonio Narro, Calzada Antonio Narro 1923, Saltillo, Coahuila, México. CP 25315. Mr. Ricardo Flores Canales is a doctoral graduate student (estudiante de Doctorado) in the Department of Parasitol-ogy, Autonomous Agricultural University Antonio Narro, 1923, Saltillo, Coahuila, México CP 20931, i.e., Departamento de Parasitología Agrícola, Universidad Autónoma Agraria Antonio Narro, Calzada Antonio Narro 1923, Saltillo, Coahuila, México. C. P. 25315.

REFERENCES CITED

1.

M. M. Abbott-Setta , and C. C. Childers 1987. A modified leaf arena technique for rearing phytoseiid or tetranychid mite for biological studies. Florida Entomol. 70: 245–248. Google Scholar

2.

L. C. Birch 1948. The intrinsic rate of natural increase of an insect population. J. Animal Ecol. 17: 15–26 Google Scholar

3.

M. Bounfour , and L. K. Tanigoshi 2001. Effect of temperature on development and demographic parameters of Tetranychus urticae and Eotetranychus carpini borealis (Acari: Tetranychidae). Ann. Entomol. Soc. America 94(3): 400–404. Google Scholar

4.

R. E. Bustamante , and H. L. F. Patiño 2001. Foro: En búsqueda de un sistema de resistencia estable en plantas cultivadas. Manejo Integrado de Plagas (Costa Rica) 60: 3–14. Google Scholar

5.

J. H. Fikru , and L. G. Higley 2003. Changes in soybean gas-exchange after moisture stress and spider mite injury. Environ. Entomol. 32: 433–440. Google Scholar

6.

J. L. Flexner , P. H. Westigar , R. Hilton , and B. A. Croft 1995. Experimental evaluation of resistance management for two-spotted spider mite (Acari:Tetranychidae) on Southern Oregon pear: 1987–1993. J. Econ. Entomol. 88: 1517–1524. Google Scholar

7.

A. E. Flores , J. Landeros , and M. H. Badii 2000. Evaluation of population parameters of Tetranychus urticae Koch (Acari:Prostigmata:Tetranychidae) exposed to avermectin. Southwestern Entomol. 25(4): 287–293. Google Scholar

8.

K. Grissa-Lebdi , I. G. Van , and P. Lebrun 2002. Demographic traits of Eotetranychus pruni from Belgian and Tunisian orchards, in comparison with Tetranychus urticae. Exp. Appl. Acarol. 26: 209–217 Google Scholar

9.

L. R. Jeppson , H. H. Keifer , and E. Baker 1975. Mites injurious to economic plants. Univ. California Press. San Francisco. 472 pp. Google Scholar

10.

N. Kheradpir , J. Khalghani , H. Ostovan , and M. R. Rezapanah 2007. The comparison of demographic traits in Tetranychus urticae Koch (Acari: Tetranychidae) on five different greenhouse cucumber hybrids (Cucumis sativus). Acta Hort. 747: 425–429. Google Scholar

11.

S. Konanz , and R. Nauen 2004. Purification and partial characterization of a glutathione S-transferase from the two-spotted spider mite, Tetranychus urticae. Pesticide Biochem. Physiol. 79: 49–57. Google Scholar

12.

J. Landeros , L. P. Guevara , M. H. Badii , A. E. Flores , and A. Pámanes 2004. Effect of different densities of the two-spotted spider mite Tetranychus urticae on CO2 assimilation, transpiration, and stomatal behaviour in rose leaves. Exp. Appl. Acarol. 32: 187–198. Google Scholar

13.

H. C. B. Li , and G. L. Hartman 2004. Effect of three resistant soybean genotype on the fecundity , mortality, and maturation of soybean aphid (Homoptera: Aphididae). J. Econ. Entomol. 97: 1106–1111. Google Scholar

14.

V. L. Maggi , and T. F. Leigh 1983. Fecundity response of the two-spotted spider mite to cotton treated with methyl parathion or phosphoric acid. J. Econ. Entomol. 76: 20–25. Google Scholar

15.

D. Marcio 2007. Sublethal effects of spirodiclofen on life history and life-table parameters of two-spotted spider mite (Tetranychus urticae). Exp. Appl. Acarol. 42: 121–129. Google Scholar

16.

J. S. Meyer , C. G. Ingersoll , L. L. McDonald , and M. S. Boyce 1996. Estimating uncertainty in population growth rates: Jacknife vs Bootstrap techniques. Ecology 67(3): 1156–1166. Google Scholar

17.

M. W. Sabelis 1991. Life history evolution in spider mites, pp. 23–49 In R. Schuster and P. W. Murphy [eds.], The Acari: Reproduction, development and life history strategies. Chapman & Hall, London. Google Scholar

18.

F. J. Sàenz De Cabezón , E. Martínez-Villar , F. Moreno , V. Marco , and I. Pérez Moreno 2006. Influence of sublethal exposure to triflumuron on the biological performance of Tetranychus urticae Koch (Acari: Tetranychidae). Spanish J. Agric. Res. 4(2): 167–172. Google Scholar

19.

E. Skorupska 1998. Morphologic-anatomical structure of leaves and demographic parameters of hawthorn spider mite, Tetranychus viennensis Zaher and the two-spotted spider mite, Tetranychus urticae Koch (Acarina: Tetranychidae) on selected scab-resistant apple varieties. J. Appl. Entomol. 122: 493–496. Google Scholar

20.

C. M. Smith 2005. Plant resistance to arthropods: molecular and conventional approaches. Springer, the Netherlands. 413 pp. Google Scholar

21.

N. Stumpf , and R. Nauen 2002. Biochemical markers linked to abamectin resistance in Tetranychus urticae (Acari: Tetranychidae). Pesticide Biochem. Physiol. 72: 111–121. Google Scholar

22.

A. P. Takematsu , N. S. Filho , M. F. De Souza Filho , and M. E. Sato 1994. Sensibilidade de Tetranychus urticae (Koch, 18S6) proveniente de roseira (Rosa sp.) de Holambra-SP a alguns acaricidas. Rev. Agric. (Piracicaba) 69(2): 129–137. Google Scholar

23.

M. Van De Vrie 1985. Greenhouse ornamentals, pp. 273–284 In W. Helle and M. W. Sabelis [eds.], Spider Mites. Their Biology, Natural Enemies and Control. Elsevier, Amsterdam, World Crop Pests, Vol 1B. Google Scholar

24.

A. Wermelinger , J. J. Oertle , and J. Baumgärner 1991. Environmental factors affecting the life-tables of Tetranychus urticae (Acari:Tetranychidae). III. Host-plant-nutrition. Exp. Appl. Acarol. 12: 259–274. Google Scholar
Jerónimo Landeros Flores, Ernesto Cerna Chávez, Luis A. Aguirre Uribe, Ricardo Flores Canales, and Yisa M. Ochoa Fuentes "Demographic Parameters of Tetranychus urticae (Acari: Tetranychidae) on Four Rosa sp. Cultivars," Florida Entomologist 96(4), 1508-1512, (1 December 2013). https://doi.org/10.1653/024.096.0432
Published: 1 December 2013
JOURNAL ARTICLE
5 PAGES


SHARE
ARTICLE IMPACT
Back to Top