The red widow spider, Latrodectus bishopi Kaston, is a species of conservation concern because it is narrowly endemic to threatened palmetto scrub found only on ancient sand ridges in peninsular Florida. We hypothesized that this spider might feed extensively on insects that also are scrub specialists. To ascertain the prey of red widow spiders, we collected arthropods trapped in webs of adult females (n = 30 per season) located in native oak-palmetto scrub at the Archbold Biological Station after dawn and before dusk for 5 consecutive days in early spring (Mar 1989) and in late spring (May 2003). We identifed a total of 42 species among the 98 specimens collected. Using published regression equations, we converted the size of each specimen to dry mass. We found that 5 species of scarab beetles endemic to Florida scrub accounted for 65% of prey by weight even though their numbers were modest (22% of prey items). In early spring red widow spiders fed predominantly on nocturnally captured coleopterans (78% of prey items), but in late spring when palmetto flowers were blooming near webs day-active hymenopterans were added to the diet. Frequency analysis showed that temporal patterns of prey capture by individual spiders were infrequent (≤ 0.4 prey per day) and statistically random.