Translator Disclaimer
1 March 2015 Reproductive Modes and Daily Fecundity of Aenasius bambawalei (Hymenoptera: Encyrtidae), a Parasitoid of Phenacoccus solenopsis (Hemiptera: Pseudococcidae)
Author Affiliations +
Abstract

Reproductive modes and daily fecundity of Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae), a parasitoid of Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae), were elucidated in this experiment. Aenasius bambawale reproduced mainly by gamogenesis and occasionally by arrhenotokous parthenogenesis. Aenasius bambawalei females allocated far more energy sources to their own survival than to reproduction during the mid and late portions of the ovipositional period. Therefore, newly emerged adult parasitoids should be chosen for mass rearing and for use in the biological control of P. solenopsis.

Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae), a solitary parasitoid of the invasive mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae), was first described and named by Hayat in India (Hayat 2009). Several investigations had shown that this parasitoid has a high parasitism rates on P. solenopsis in India (Sharma 2007; Tanwar et al. 2008; Mohindru et al. 2009). In India, various studies focused on observing the parasitization efficiency of A. bambawalei under the laboratory conditions, and natural parasitism of P. solenopsis by the parasitoid in the field (Kumar et al. 2009; Jhala et al. 2009; Prasad et al. 2011; Sankar et al. 2011). Subsequently A. bambawalei was also discovered in Pakistan (Ashfaq et al. 2010; Bodlah et al. 2010) and China (Chen et al. 2010). Previous laboratory experiments have demonstrated that A. bambawalei prefers to parasitize the P. solenopsis 3rd instars, and the 3rd instars are also best fit for the parasitoid's development, progeny fitness and favorable sex ratio (Fand et al. 2011; He et al. 2012).

The most typical reproductive mode of parasitoids is haplodiploidy, in which unfertilized eggs develop into males and fertilized eggs into females. However, another reproductive mode in some parasitoid species is thelytoky in which unfertilized eggs can produce female offspring (e.g., Wenseleers & Billen 2000; Giorgini et al. 2010; Rabeling & Kronauer 2013). In our experiment, reproductive modes and daily fecundity of A. bambawalei were observed, the results provide useful information for understanding the reproductive behavior and for utilizing the parasitoid.

Phenacoccus solenopsis Tinsley were collected from Hibiscus rosasinensis L. (Malvales: Malvaceae) plants on the campus of South China Agricultural University (SCAU), Guangzhou, Guangdong Province, China. We fed these specimens on 10 cm-tall seedlings of potato (Solanum tuberosum L. (Solanales: Solanaceae) planted in 7.5 cm diam plastic pots. Subsequently, the 1st instars were placed on leaves of the potted H. rosa-sinensis plants and raised for several generations. Aenasius bambawalei Hayat wasps were also collected from the campus of SCAU. Thus, parasitized mealybug nymphs were collected from H. rosa-sinensis plants, and then taken to the laboratory, where they were cultured. Parasitoids that emerged from mummified mealybugs were identified and raised for several generations. Third instar mealybug nymphs and newly emerged adults were used for experiments. The mealybug and parasitoid populations were reared in the laboratory at 27 ± 1 °C and 60 – 70% RH. A 10% solution of honey mixed with purified water was supplied for parasitoid adults.

Each mated A. bambawalei female was transferred to a group of 30 third instar mealybugs on fresh 10 cm-tall potted S. tuberosum seedlings in a clean transparent cylinder (7.5 × 11 cm) with a 10% honey solution for 24 h. The experiment was conducted at 27 ± 1 °C, 70 ± 5% RH and 12:12 h L:D. After 24 h, the parasitoids were removed while the exposed host nymphs remained on the S. tuberosum seedlings. The exposed nymphs were checked daily until adult parasitoids emerged from the mummified mealybugs. Number and sex ratio of the parasitoids were recorded, and hind-tibia lengths of males and females were measured for delimiting body sizes. The experiment was replicated 20 times. A parallel experiment was also conducted with unmated parasitoid females.

Each mated female was then provided with 40 host nymphs on fresh potted seedlings of S. tuberosum in a cylinder as described above. These circular containers with host nymphs feeding on fresh potted S. tuberosum seedlings were held at 27 ± 1 °C, 70 ± 5% RH and 12:12 h L:D. Each day the number and the survival of the offspring were recorded when parasitoid adults emerged from mummified mealybugs. Females were fed daily with 10% fresh honey solution during the experiment.

Data were checked for normality and homoscedasticity before comparison analysis, analyzed by one-way ANOVA and multiple comparisons of means were conducted by the least significant difference (LSD) test (SAS Institute 2004).

We found that mated parasitoid females could oviposit fertilized eggs within 24 h. Their offspring included males and females, and the female fraction of the progeny adults was 0.9. Unmated parasitoid females could oviposit unfertilized eggs within 24 h, but all their offspring was males. The mean generation (T) times of female and male progeny of mated parasitoid females were 14.4 days and 13.4 days, respectively. In contrast the mean generation (T) time of male progeny of unmated parasitoid females was 16.4 days, which was significantly longer than the mean generation time of male progeny of mated parasitoid females (F1,38 = 207.05, P < 0.0001) (Table 1). In addition, the parasitism rate (Table 1; F1,38 = 25.30, P < 0.0001) and the hind-tibia lengths of male progeny (Table 1; F1,38 = 41.15, P < 0.0001) of the mated females were both significantly higher than the corresponding values pertaining to unmated females. This implies that copulation can be beneficial for the fitness of the parasitoid females. In many parasitoid species the daily production of progeny (fecundity) fluctuates and shows multiple peaks during female lifespans, and females of these parasitoid species can repeatedly mate (e.g., Muegge & Lambdin 1989; Baezalarios et al. 2002; Karamaouna & Copland 2009; Zipporah et al. 2013). We found that A. bambawalei adult females could survive 77 days. The oviposition peak occurred on the 2nd day, and then daily fecundity decreased sharply when females had mated only once (Fig. 1). The female ovipositional period was 21 days. This implies that A. bambawalei females may allocate more energy resources to survival than to reproduction during the mid and late ovipositional periods if they mated once. Previous studies showed that increasing mating frequency increases female fitness parameters (Arnqvist & Nilsson 2000; Fox & Rauter 2003; Avila et al. 2011) such as longevity and fecundity (Savalli & Fox 1999). Would the daily production of progeny by A. bambawalei females display multiple peaks if mate multiple times? Future work is needed to answer this question. So far, regrettably, there is no evidence that A. bambawalei females will mate more than once.

Table 1.

Developmental parameters of Aenasius bambawalei progeny that were the result of sexual (haplodiploidy) vs asexual (arrhenotoky) reproduction.

t01_358.gif

Fig. 1.

Daily survival rate and daily fecundity of Aenasius bambawalei females.

f01_358.jpg

References Cited

  1. G Arnqvist , T Nilsson. 2000. The evolution of polyandry: multiple mating and female fitness in insects. Animal Behaviour 60: 145–164. Google Scholar

  2. M Ashfaq , GS Shah , AR Noor , SP Ansari , S Mansoor. 2010. Report of a parasitic wasp (Hymenoptera: Encyrtidae) parasitizing cotton mealybug (Hemiptera: Pseudococcidae) in Pakistan and use of PCR for estimating parasitism levels. Biocontrol Science and Technology 20: 625–630. Google Scholar

  3. FW Avila , LK Sirot , BA LaFlamme , CD Rubinstein , MF Wolfner. 2011. Insect seminal fluid proteins: Identification and function. Annual Review of Entomology 56: 21–40. Google Scholar

  4. G Baezalarios , J Sivinski , T Holler , M Aluja. 2002. The effects of chilling on the fecundity and life span of mass-reared parasitoids (Hymenoptera: Braconidae) of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Biocontrol Science and Technology 12: 205– 215. Google Scholar

  5. I Bodlah , M Ahmad , MF Nasir , M Naeem. 2010. Record of Aenasius bambawalei Hayat, 2009 (Hymenoptera: Encyrtidae), a parasitoid of Phenacoccus solenopsis (Sternorrhyncha: Pseudococcidae) from Punjab, Pakistan. Pakistan Journal of Zoology 42: 533–536. Google Scholar

  6. HY Chen , RX Cao , ZF Xu. 2010. First record of Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae), a parasitoid of the mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) from China. Journal of Environmental Entomology 32: 293–295. Google Scholar

  7. M Giorgini , U Bernardo , MM Monti , AG Nappo , M Gebiola. 2010. Rickettsia symbionts cause parthenogenetic reproduction in the parasitoid wasp Pnigalio soemius (Hymenoptera: Eulophidae). Appled and Environmental Microbiology 76: 2589–2599. Google Scholar

  8. BB Fand , RD Gautam , SS Suroshe. 2011. Suitability of various stages of mealybug, Phenacoccus solenopsis (Homoptera: Pseudococcidae) for development and survival of the solitary endoparasitoid, Aenasius bambawalei (Hymenoptera: Encyrtidae). Biocontrol Science and Technology 21: 51–55. Google Scholar

  9. CW Fox , CM Rauter. 2003. Bet-hedging and the evolution of multiple mating. Evolutionary Ecology Research 5: 273–286. Google Scholar

  10. M Hayat. 2009. Description of a new species of Aenasius Walker (Hymenoptera: Eneyrtidae), parasitoid of the mealybug, Phenacoccus solenopsis Tinsley in India. Biosystematica 3: 21–26. Google Scholar

  11. LF He , DD Feng , P Li , ZF Xu. 2012. Host-instar selection of Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae) for mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Phenacoccidae). Journal of Environmental Entomology 34: 329–333. Google Scholar

  12. RC Jhala , RF Solanki , TM Bharpoda , MG Patel. 2009. Occurrence of hymenopterous parasitoids, Aenasius bambawalei Hayat and Promuscidea unfaciativentris Girault on cotton mealybugs, Phenacoccus solenopsis Tinsley in Gujarat. Insect Environment 14: 164–165. Google Scholar

  13. F Karamaouna , MJ Copland. 2009. Fitness and life history parameters of Leptomastix epona and Pseudaphycus flavidulus, two parasitoids of the obscure mealybug Pseudococcus viburni. BioControl 54: 65–76. Google Scholar

  14. R Kumar , KR Kranthi , D Monga , SL Jat. 2009. Natural parasitization of Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) on cotton by Aenasius bambawlei Hayat (Hymenoptera: Encyrtidae). Journal of Biological Control 23: 457–460. Google Scholar

  15. B Mohindru , V Jindal , AK Dhawan. 2009. Record of parasitoid on mealybug Phenacoccus solenopsis in tomato. Indian Journal of Ecology 36: 101–102. Google Scholar

  16. MA Muegge , PL Lambdin. 1989. Longevity and fecundity of Coccophacus lycimnia (Walker) (Hymenoptera: Aphelinidae), a primary parasitoid of Coccus hesperjdum (Homoptera: Coccidae). Journal of Agricultural Entomology 6: 169–174. Google Scholar

  17. YG Prasad , M Prabhakar , G Sreedevi , M Thirupathi. 2011. Spatio-temporal dynamics of the parasitoid, Aenasius bambawalei Hayat (Hymenoptera: Encyrtidae) on mealybug, Phenacoccus solenopsis Tinsley in cotton based cropping systems and associated weed flora. Journal of Biology Control 25: 198–202. Google Scholar

  18. C Rabeling , DJC Kronauer. 2013. Thelytokous parthenogenesis in eusocial Hymenoptera. Annual Review of Entomology 58: 273–292. Google Scholar

  19. C Sankar , R Marimuthu , P Saravanan , P Jeyakumar , RK Tanwar , S Sathyakumar , O M Bambawale , VV Ramamurthy , B Anupam. 2011. Predators and parasitoids of cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) in Perambalur district of Tamil Nadu. Journal of Biological Control 25: 242–245. Google Scholar

  20. SAS Institute. 2004. SAS User's® Guide: Statistics. SAS Institute, Cary, NC. Google Scholar

  21. UM Savalli , CW Fox. 1999. The effect of male mating history on paternal investment, fecundity and female remating in the seed beetle Callosobruchus maculatus. Functional Ecology 13: 169–177. Google Scholar

  22. SS Sharma. 2007. Aenasius sp. nov. effective parasitoid of mealybug (Phenacoccus solenopsis) on okra. Haryana Journal of Horticultural Sciences 36: 412. Google Scholar

  23. RK Tanwar , VK Bhamare , VV Ramamurthy , M Hayat , P Jeyakumar , A Singh , OM Bambawale. 2008. Record of new parasitoids on mealybug, Phenacoccus solenopsis. Indian Journal of Entomology 70: 404–405. Google Scholar

  24. T Wenseleers , J Billen. 2000. No evidence for Wolbachia-induced parthenogenesis in the social Hymenoptera. Journal of Evolutionary Biology 13: 277–280. Google Scholar

  25. O Zipporah , G Linus , M Shadrack , M Samuel , S Srinivasan. 2013. Influence of mating frequency and parasitoid age on reproductive success of Trichogrammatoidea sp. nr. lutea Girault collected from Plutella xylostella Linnaus in Kenya. International Journal of Agricultural Sciences 3: 114–123. Google Scholar

Lang-Fen He, Dong-Dong Feng, Pan Li, Zhong-Shi Zhou, and Zai-Fu Xu "Reproductive Modes and Daily Fecundity of Aenasius bambawalei (Hymenoptera: Encyrtidae), a Parasitoid of Phenacoccus solenopsis (Hemiptera: Pseudococcidae)," Florida Entomologist 98(1), (1 March 2015). https://doi.org/10.1653/024.098.0158
Published: 1 March 2015
JOURNAL ARTICLE
3 PAGES


SHARE
ARTICLE IMPACT
Back to Top