Leafhoppers (Hemiptera: Cicadellidae) that inhabit edge grasses throughout the winter were tested for the presence of corn stunt spiroplasma (CSS) and maize bushy stunt phytoplasma (MBSP). Leafhoppers were sampled using a sweep net at least once a week between Feb and Jun 2014 in Jalisco, Mexico. In total, 2,263 leafhoppers from 27 different taxa were collected. Stirellus bicolor Van Duzee and Graminella sonora (Ball) were the most abundant leafhoppers, with smaller numbers of Dalbulus maidis Delong and D. elimatus Ball. None of the leafhoppers tested positive for CSS or MBSP, suggesting that these 2 bacterial pathogens do not overwinter in leafhoppers that live on edge grasses. This is an important finding, because several studies have found that crop edges are beneficial reservoirs of insect predators and parasitoids, including those that attack insect vectors of plant pathogens.
Field margins (edges) in certain crops are important because they form habitats that maintain herbivorous insects and their parasitoids and predators (Ramsden et al. 2015). However, little is known about whether these herbivores carry plant pathogens, such as bacteria that can infest the crops. In Mexico, 97% of maize (Zea mays L. ssp. mays; Poales: Poaceae) is planted annually during the maize-growing wet season (Jun to Oct) (Moya-Raygoza et al. 2004). Once the maize dries out, green grasses that grow on the edges of the maize fields serve as food resources for the overwintering insects. These monocots are colonized by herbivorous leafhoppers (Hemiptera: Cicadellidae) capable of harboring viral and bacterial pathogens of maize plants, leading to concerns that they could transmit these pathogens to crops during the next maize-growing wet season (Moya-Raygoza et al. 2007). The bacterial pathogens corn stunt spiroplasma (CSS), Spiroplasma kunkelii Whitcomb et al. (Entomoplasmatales: Spiroplasmataceae), and maize bushy stunt phytoplasma (MBSP), ‘Candidatus Phytoplasma asteris’ (Acholeplasmatales: Acholeplasmataceae), are transmitted efficiently by the leafhoppers Dalbulus maidis Delong and D. elimatus Ball, both of which are important pests of maize in Latin America (Nault 1990). Furthermore, other leafhoppers such as Graminella nigrifrons Forbes, Stirellus bicolor Van Duzee, and Exitianus exitiosus (Uhler) are also vectors of CSS and/or MBSP (Nault 1980). The objective of this study was therefore to determine whether or not leafhoppers that inhabit edge grasses carry either of the pathogens (CSS or MBSP).
The study was conducted in the region of Zapopan, state of Jalisco, Mexico (20°44′N, 103°30′W; 1,662 m). Sampling was conducted on fallow perennial grasses of the family Poaceae; Brachiaria brizantha (A. Richard) Stapf, Chloris gayana Kunth, Cynodon dactylon (L.) Persoon, Melinis minutiflora P. Beauvois, Panicum maximum Jacquin, and Rhynchelytrum repens (Willdenow). All grasses showed green foliage during the sampling period. Leafhopper adults were collected from the grasses, using a sweep net, at least once a week during the winter (dry season), from Feb to Jun 2014. Sweep net collections of leafhoppers were conducted for an hour between 11 a.m. and 12 p.m., performing 1,800 sweeps on each sampling date. The collected leafhoppers were maintained in 95% ethanol for future identification and DNA extraction. Leafhoppers were identified to genus or species level using keys and previously identified leafhoppers for comparison. Voucher specimens were deposited in the entomological collections of the University of Guadalajara, Jalisco, Mexico.
DNA was extracted from each individual leafhopper by using the protocol developed by Aljanabi & Martinez (1997). CSS was detected by polymerase chain reaction (PCR) amplification of the CSS spiralin gene, following the method of Barros et al. (2001). Previously extracted CSS DNA was included in each gel as a positive control. MBSP was detected by PCR amplification of the phytoplasma 16S rRNA gene from the leafhopper DNA extracts. First, we used the universal primer pairs P1 (Deng & Hiruki 1991) and Tint (Smart et al. 1996) using the PCR conditions described by Smart et al. (1996) to obtain preliminary results. We also used the leafhopper DNA extracts as the template for a nested PCR assay. Universal primer pairs P1 (Deng & Hiruki 1991) and P7 (Schneider et al. 1995) were used for the first reaction, and R16F2n/ R16R2 (Gundersen & Lee 1996) for the nested reaction. In both cases, besides the leafhopper DNA extract, we used DNA extract from macadamia trees affected by ‘Candidatus Phytoplasma asteris’ (Pérez-López et al. 2013) as positive controls. The PCR products were run on a 1.0% agarose gel for 1 h at 80 V and stained with ethidium bromide to visualize the positive bands for CSS (600 bp) and MBSP (1,600 bp and 1,250 bp for the P1/Tint and nested PCR reactions, respectively).
In total, we collected 2,263 leafhopper individuals belonging to 27 taxa. The most abundant species were S. bicolor and Graminella sonora (Ball) (Table 1). All collected taxa were negative for CSS and MBSP during the winter. This suggests that leafhoppers that inhabit edge perennial grasses, at least in the sampled area during winter, do not harbor CSS or MBSP.
CSS and MBSP are transmitted by D. maidis, D. elimatus, and G. nigrifrons, whereas CSS, but not MBSP, is transmitted by S. bicolor, E. exitiosus (Nault 1980), and Exitianus obscurinervis Stål (Carloni et al. 2011). We found D. maidis, D. elimatus, G. sonora, and S. bicolor on green edge grasses during winter, but none were positive for CSS or MBSP. This result has important implications for edge management because it suggests that although winter grasses maintain potential insect vectors, these vectors are not infected with CSS or MBSP.
Abundance of leafhoppers species on edge grasses throughout winter 2014 in Jalisco, Mexico.
An analysis of body size differences (Moya-Raygoza et al. 2007, 2012) suggested that D. maidis and D. elimatus adults can move both locally and over long distances. Dalbulus maidis adults infected with CSS could have migrated to high-elevation sites such as Zapopan from low-elevation sites (less than 1,000 m) elsewhere in Mexico where maize is cultivated (Moya-Raygoza et al. 2007). Also, D. maidis infected with CSS can overwinter on volunteer maize. For example, CSS was recovered from D. maidis throughout the winter from volunteer maize in California, USA (Summers et al. 2004). No studies have investigated the overwinter presence of MBSP in D. maidis or D. elimatus in natural conditions. However, it is known that maize plants with MBSP symptoms are found at elevations higher than 1,000 m (Nault 1990). Previous experimental studies have demonstrated that CSS and MBSP are limited to Zea species, including maize and its teosinte relatives, whereas other Poaceae such Tripsacum, Coix, and Sorghum species, do not harbor either bacterial pathogen (Nault 1980).
Edge grasses are important, positive habitats because they maintain parasitoids of D. maidis. Recently, in the same region of Zapopan, Moya-Raygoza & Becerra-Chiron (2014) found that eggs of D. maidis were parasitized by Oligosita sp. (Hymenoptera: Trichogrammatidae) and Anagrus columbi Perkins (Hymenoptera: Mymaridae) throughout the 2012-2013 winter in perennial grasses habitat. Also in that habitat, dryinids (Hymenoptera) and strepsipterans parasitized the most abundant leafhoppers inhabiting edge grasses in winter, including S. bicolor, G. sonora, Exitianus picatus Gibson, Amblysellus sp., and D. maidis. In conclusion, the results reported here suggest that leafhoppers that inhabit edge grasses during winter in Zapopan do not harbor CSS or MBSP. On the other hand, past studies have reported that edges are reservoirs of natural enemies of leafhoppers. Future studies examining the presence of CSS and MBSP in leafhoppers that live on edge grasses during different growing seasons and in multiple locations would help confirm this finding.
We thank Christopher Dietrich (Illinois National History Survey) and Jorge Adilso Pinedo-Escatel for confirming the identification of some leafhopper taxa. We also thank Veronica Palomera-Avalos and Aaron Rodriquez-Contreras for insightful and useful advice and laboratory support for the CSS detection experiments. Edel Pérez-López received financial support from CONACYT for the MBSP detection experiments (CVU: 517835). Also, we thank Claudia S. Copeland (Carpe Diem Biomedical Writing and Editing) for editing the manuscript.
- SM Aljanabi , I Martinez. 1997. Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Research 25: 4692–4693. Google Scholar
- TSL Barros , RE Davis , RO Resende , EL Dally. 2001. Design of a polymerase chain reaction for specific detection of corn stunt spiroplasma. Plant Disease 85: 475–480. Google Scholar
- E Carloni , E Virla , S Paradell , P Carpane , C Nome , I Laguna , MP Giménez Pecci. 2011. Exitianus obscurinervis (Hemiptera: Cicadellidae), a new experimental vector of Spiroplasma kunkelii. Journal of Economic Entomology 104: 1793–1799. Google Scholar
- S Deng , C Hiruki. 1991. Amplification of 16S rRNA genes from culturable and non-culturable mollicutes. Journal of Microbiological Methods 14: 53–61. Google Scholar
- DE Gundersen , IM Lee. 1996. Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer sets. Phytopathologia Mediterranea 35: 144–151. Google Scholar
- G Moya-Raygoza , IM Becerra-Chiron. 2014. Overwintering biology of egg parasitoids of Dalbulus maidis (Hemiptera: Cicadellidae) on perennial grasses, volunteer maize, stubble, and drip-irrigated maize. Annals of the Entomological Society of America 107: 926–932. Google Scholar
- G Moya-Raygoza , J Kathirithamby , KJ Larsen. 2004. Dry season parasitoids of adult corn leafhoppers (Hemiptera: Cicadellidae) on irrigated maize in Mexico. Canadian Entomologist 136: 119–127. Google Scholar
- G Moya-Raygoza , V Palomera-Avalos , C Galaviz-Mejia. 2007. Field overwintering biology of Spiroplasma kunkelii (Mycoplasmatales: Spiroplasmataceae) and its vector Dalbulus maidis (Hemiptera: Cicadellidae). Annals of Applied Biology 151: 373–379. Google Scholar
- G Moya-Raygoza , A Muñoz Urias , CA Uribe-Mu. 2012. Habitat, body size and reproduction of the leafhopper, Dalbulus elimatus (Hemiptera: Cicadellidae), during the winter dry season. Florida Entomologist 95: 382–386. Google Scholar
- LR Nault. 1980. Maize bushy stunt and corn stunt: a comparison of disease symptoms, pathogen host ranges, and vectors. Phytopathology 70: 659–662. Google Scholar
- LR Nault. 1990. Evolution of an insect pest: maize and the corn leafhopper, a case of study. Maydica 34: 163–175. Google Scholar
- E Pérez-López , L Hernández-Rodríguez , ML Pantoja , MRH Zaldívar. 2013. First report of a ‘Candidatus Phytoplasma asteris’ isolate affecting macadamia nut trees in Cuba. New Disease Reports 28: 1. Google Scholar
- MW Ramsden , R Mendéz , SR Leather , F Wäckers. 2015. Optimizing field margins for biocontrol services: the relative role of aphid abundance, annual floral resources, and overwinter habitat in enhancing aphid natural enemies. Agriculture, Ecosystems and Environment 199: 94–104. Google Scholar
- B Schneider , M Cousin , S Klinkong , E Seemüller. 1995. Taxonomic relatedness and phylogenetic positions of phytoplasmas associated with diseases of faba bean, sunn hemp, sesame, soybean and eggplant. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 102: 225–232. Google Scholar
- CD Smart , B Schneider , CL Blomquist , LJ Guerra , NA Harrison , U Ahrens , KH Lorenz , E Seemüller , BC Kirkpatrick. 1996. Phytoplasma-specific PCR primers based on sequences of the 16S-23S rRNA spacer region. Applied and Environmental Microbiology 62: 2988–2993. Google Scholar
- CG Summers , Jr AS Newton , DC Opgenorth. 2004. Overwintering of corn leafhopper, Dalbulus maidis (Homoptera: Cicadellidae), and Spiroplasma kunkelii (Mycoplasmatales: Spiroplasmataceae) in California's San Joaquin Valley. Environmental Entomology 33: 1644–1651. Google Scholar